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| Status |
Public on Jul 07, 2009 |
| Title |
High-throughput pyrosequencing of miRNAs from Medicago truncatula nodules and roots |
| Organism |
Medicago truncatula |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
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| Summary |
We have used deep sequencing of small RNAs from nodules and root apexes of the model legume Medicago truncatula, to identify 113 novel candidate miRNAs. These miRNAs (legume or Mt-specific) are encoded by 278 putative hairpin precursors in the M. truncatula genome. Several miRNAs are differentially expressed in nodules and root tips and large variety of targets could be predicted for these genes. Specific miRNA isoforms showed contrasting expression patterns in these tissues
Keywords: Transcriptome analysis
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| Overall design |
3 samples examined: nodules, root tips, and root tips + NaCl
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| Contributor(s) |
Lelandais C, Naya L, Sallet E, Calenge F, Frugier F, Hartmann C, Gouzy J, Crespi M |
| Citation(s) |
19767456 |
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| Submission date |
Mar 27, 2009 |
| Last update date |
May 15, 2019 |
| Contact name |
Jerome Gouzy |
| E-mail(s) |
[email protected]
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| URL |
http://lipm-bioinfo.toulouse.inra.fr
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| Organization name |
INRA/CNRS
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| Department |
LIPM
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| Street address |
24 Chemin de Borde Rouge, BP52627
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| City |
Castanet Tolosan |
| ZIP/Postal code |
31326 |
| Country |
France |
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| Platforms (1) |
| GPL9322 |
454 GS FLX (Medicago truncatula) |
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| Samples (3) |
| GSM387472 |
small RNAs from Medicago truncatula, Jemalong A17, Mature nodules |
| GSM387473 |
small RNAs from Medicago truncatula, Jemalong A17, Root tips |
| GSM387474 |
small RNAs from Medicago truncatula, Jemalong A17, Root tips + NaCl 100 mM 1 hour |
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| Relations |
| SRA |
SRP001018 |
| BioProject |
PRJNA115799 |
| Supplementary data files not provided |
SRA Run Selector |
| Processed data included within Sample table |
| Raw data are available in SRA |
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