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| Status |
Public on Sep 18, 2020 |
| Title |
Reduced Representational Bisulfite Sequencing of R1 and Dnmt1 Tet/Tet mouse embryonic stem cell lines |
| Organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
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| Summary |
We generated RRBS data to enable a comparative DNA methylation analysis of Dnmt1 Tet/Tet, a mouse embyonic stem cell line (ESC) that overexpresses DNMT1 and the WT R1 ESCs and their neuronal derivatives. The optimized data analysis workflows provides a framework for comparative investigations of methylation profiles. This platform offers a comprehensive and more accurate quantitative and qualitative evaluation of methylated cytosines in ESCs and neurons. We expect that RRBS-based methylome analysis permits to understand the epigenetic basis of abnormal neurogenesis.
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| Overall design |
Study of the methylation profiles of R1 and Dnmt1 Tet/Tet ESCs and their neuronal derivatives.
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| Contributor(s) |
Mohan KN, Saxena S, Choudhury S |
| Citation(s) |
32944600 |
|
https://doi.org/10.1016/j.dib.2020.106242
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| |
| Submission date |
Jun 19, 2020 |
| Last update date |
Sep 21, 2020 |
| Contact name |
Kommu Naga Mohan |
| E-mail(s) |
[email protected]
|
| Phone |
4066303608
|
| Organization name |
Birla Institute of Technology, Pilan- Hyderabad Campus
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| Department |
Biological Sciences
|
| Lab |
Mohan Laboratory
|
| Street address |
shameerpet, Jawaharnagar
|
| City |
Hyderabad |
| State/province |
Telangana |
| ZIP/Postal code |
500078 |
| Country |
India |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA640537 |
| SRA |
SRP267969 |
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