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| Status |
Public on Jan 13, 2022 |
| Title |
Single cell RNA sequencing on PyMT mammary gland tumor cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We labeled PyMT control cells versus PyMT-GPx2 KD with GFP in vitro and then injected into mammary fat pad of mice for incubating 45 days. We then generated single cell suspension by FACS sorting from PyMT-control, GPx2 KD. 10X Genomics was used to make cDNA library. We then sequenced the samples with Illumina high throughput sequencing.
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| Overall design |
Investigating how GPx2 loss affects mammary tumor heterogeneity and metastasis.
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| Contributor(s) |
Ren Z, Hazan RB, Dharmaratn M, Kulkarni AS, Mar JC, Taherian Fard A |
| Citation(s) |
35193955, 38238426 |
| |
| Submission date |
Jun 12, 2020 |
| Last update date |
Apr 02, 2024 |
| Contact name |
Rachel Hazan |
| Phone |
7184303374
|
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Pathology
|
| Lab |
Forcheimer Rm. 526
|
| Street address |
1300 Morris Park Avenue
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA639108 |
| SRA |
SRP267089 |
