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| Status |
Public on Jun 12, 2020 |
| Title |
Sustained activation of AMPK enhances differentiation of human iPSC-derived cardiomyocytes via sirtuin activation |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Lee and colleagues demonstrate that sustained activation of AMPK enhances differentiation of iPSC-derived cardiomyocytes. Sustained AMPK activation decreased histone acetylation at known target sites for nuclear-localized sirtuins, suggesting that AMPK activation enhances sirtuin activity. AMPK-induced sirtuin-mediated deacetylation of histone proteins may regulate chromatin accessibility and enhance cardiomyocyte differentiation.
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| Overall design |
We have done the experiment in duplicate for NAM and AICAR treated iPSC-derived Cardiomyoytes.
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| |
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| Contributor(s) |
Sarikhani M, Lee RT |
| Citation(s) |
32649901 |
| |
| Submission date |
Jun 11, 2020 |
| Last update date |
Aug 24, 2020 |
| Contact name |
Mohsen Sarikhani |
| E-mail(s) |
[email protected]
|
| Phone |
8578560063
|
| Organization name |
Harvard University
|
| Department |
Stem cell and regenerative biology
|
| Lab |
Lee
|
| Street address |
7 Divinity,Fairchild 159
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| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
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| Platforms (1) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA638838 |
| SRA |
SRP266919 |
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