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| Status |
Public on Nov 09, 2022 |
| Title |
Potential modulation of Puf3 chromatin association mediated by Rpb4 |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Gene expression involving RNA polymerase II is regulated by the concerted interplay between mRNA synthesis and degradation, a crosstalk where mRNA decay machinery impacts transcription and transcription machinery influences mRNA stability. Rpb4, and likely the dimer Rpb4/7 seem to be the central components of the RNA pol II governing these processes. Here we investigate the effect of ∆rrp4 on the potential chromatin association of the RNA binding protein Puf3 to chromatin in S. cerevisiae.
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| Overall design |
ChIP-seq was performed for 8 samples: 3 biological replicates for PUF3-TAP WT and PUF3-TAP Δrpb4 strains, and 2 biological replicates for the WT strain. For each sample, input was also sequenced.
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| Contributor(s) |
Alekseenko A, Pelechano V, Garrido-Godino AI, Gupta I, Gutierrez-Santiago F, Martinez-Padilla AB, Alekseenko A, Steinmetz LM, Perez-Ortin JE, Pelechano V, Navarro F |
| Citation(s) |
33094674 |
| |
| Submission date |
Jun 09, 2020 |
| Last update date |
Feb 09, 2023 |
| Contact name |
Vicent Pelechano |
| E-mail(s) |
[email protected]
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| Organization name |
ScilifeLab - Karolinska Institutet
|
| Department |
MTC
|
| Street address |
Nobels väg 16
|
| City |
Solna |
| ZIP/Postal code |
SE-17177 |
| Country |
Sweden |
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| Platforms (1) |
| GPL19756 |
Illumina NextSeq 500 (Saccharomyces cerevisiae) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA638298 |
| SRA |
SRP266591 |
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