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| Status |
Public on Nov 24, 2020 |
| Title |
Round 2 RNA-sequencing analysis of the OsIMA overexpression and knockdown rice lines and Fe deficiency responsiveness of non-transformant (NT) rice. |
| Organism |
Oryza sativa |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the comparison of transcript expression of Fe-sufficient roots of OsIMA overexpression lines IMA1OX-4, IMA2OX-1, OsIMA knockdown lines IMA1i-2, IMA2i-3, and corresponding NT, as well as Fe-deficient roots of NT, by RNA-Seq.
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| Overall design |
Total RNA was extracted from Fe-sufficient roots of OsIMA overexpression lines IMA1OX-4, IMA2OX-1, OsIMA knockdown lines IMA1i-2, IMA2i-3, and corresponding NT, as well as Fe-deficient roots of NT, and used for RNA-Seq library preparation, sequencing, mapping and quantification of each gene expression as read per million (RPM). Fe deficiency responses in NT was analyzed by the expression ratiios of NT1 -Fe/NT1 +Fe and NT3 -Fe/NT3 +Fe.
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| Contributor(s) |
Kobayashi T, Nagano AJ |
| Citation(s) |
33206982 |
| |
| Submission date |
Jun 06, 2020 |
| Last update date |
Feb 23, 2021 |
| Contact name |
Takanori Kobayashi |
| E-mail(s) |
[email protected]
|
| Phone |
+81 76 227 7505
|
| Organization name |
Ishikawa Prefectural University
|
| Department |
Research Institute for Bioresources and Biotechnology
|
| Street address |
1-308 Suematsu
|
| City |
Nonoichi |
| State/province |
Ishikawa |
| ZIP/Postal code |
921-8836 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL19290 |
Illumina HiSeq 2500 (Oryza sativa) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE151941 |
RNA-sequencing analysis of the OsIMA overexpression and knockdown rice lines and Fe deficiency responsiveness of non-transformant (NT) rice |
|
| Relations |
| BioProject |
PRJNA637775 |
| SRA |
SRP266246 |
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