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| Status |
Public on Jun 18, 2020 |
| Title |
mRNA sequencing of human osteoarthritis chondrocytes treated with or without autotaxin inhibitor |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
OBJECTIVE: To identify the changes in gene expression elicted by ATX inhibition in human OA primary chondrocytes. Chondrocytes were obtained from patients undergoing knee artheroplasty, cultured, and treated for 24 h with ATX inhibitor or control (without inhibitor). Total RNA was then extracted, and mRNA libraries were prepared using Illumina's TruSEQ stranded mRNA library preparation kit, pooled together, and sequenced on Illumina's NextSeq550.
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| Overall design |
Differential expression analysis was performed on the mRNA-SEQ data obtained from 6 prepared libraries, where 3 were treated with the ATX inhibitor. Subsequent pathway analysis identified pathways related to cholesterol analogue metabolism and cell-cycle that could be modulated by ATX inhibition in human OA chondrocytes.
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| Contributor(s) |
Datta P, Gandhi R, Nakamura S, Lively S, Rossomacha E, Potla P, Shestopaloff K, Endisha H, Pastrello C, Jurisica I, Rockel JS, Kapoor M |
| Citation(s) |
36474685 |
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| Submission date |
Jun 06, 2020 |
| Last update date |
Jan 04, 2023 |
| Contact name |
Mohit Kapoor |
| E-mail(s) |
[email protected]
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| Organization name |
Schroeder Arthritis Institute
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| Street address |
60 Leonard Ave
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| City |
Toronto |
| State/province |
Ontario |
| ZIP/Postal code |
M5T 0S8 |
| Country |
Canada |
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| Platforms (1) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA637770 |
| SRA |
SRP266247 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE151937_PDatta_mRNAseq_genecounts.tsv.gz |
809.6 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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