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| Status |
Public on May 23, 2020 |
| Title |
Transcriptome analysis of cucumber in response to high temperature stress |
| Organism |
Cucumis sativus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In order to find out the genes involved in the heat shock response of cucumber, the seedlings of '9930', a North China type cucumber variety, were treated with high temperature at 42℃, and the leaves of the seedlings were taken after 0, 3 and 6 hours of treatment for transcriptome sequencing to analyze the differential expression genes of cucumber in response to high temperature stress. The results showed that compared with the control (heat treatment at 0h), there were 6 082 genes differentially expressed after heat treatment for 3 h and 6 h, of which 3245 were up-regulated and 2 388 were down-regulated. The results of enrichment analysis of GO and KEGG showed that these differentially expressed genes were mainly involved in metabolic pathway and protein synthesis pathway. Among the differentially expressed genes, there are many Hsp family genes, including Hsp20, HSP70 and Hsp90, which are up-regulated by heat stress, indicating that these genes may play a positive regulatory role in heat shock response of cucumber. In addition, many transcription factors, including AP2, MYB, WRKY, bHLH and HSF, were also induced by heat.
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| Overall design |
mRNA profile of cucumber after heat treatment for 0,3,6 hours
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| Contributor(s) |
Ren Z, Chen X, Han J |
| Citation(s) |
32977756 |
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| Submission date |
May 22, 2020 |
| Last update date |
Oct 02, 2020 |
| Contact name |
Zhonghai Ren |
| E-mail(s) |
[email protected]
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| Organization name |
Shandong Agricultural University
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| Department |
College of Horticulture Science and Engineering
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| Lab |
State Key Laboratory of Crop Biology
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| Street address |
Daizong Road No.61,Taian
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| City |
Shandong |
| ZIP/Postal code |
271018 |
| Country |
China |
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| Platforms (1) |
| GPL26594 |
Illumina NovaSeq 6000 (Cucumis sativus) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA634519 |
| SRA |
SRP262962 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE151055_RAW.tar |
8.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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