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Series GSE15078 Query DataSets for GSE15078
Status Public on Mar 05, 2009
Title Fog2 regulation of gene expression in the adult heart
Organism Mus musculus
Experiment type Expression profiling by array
Summary MHCaCre induced knockout of Fog2flox.
Aberrant transcriptional regulation contributes to the pathogenesis of both congenital and adult forms of heart disease. While the transcriptional regulator FOG2 is known to be essential for heart morphogenesis and coronary development, its tissue specific function has not been previously investigated. Additionally, little is known about the role of FOG2 in the adult heart. Here we use spatiotemporally regulated inactivation of Fog2 to delineate its function both in embryo and adult heart. Early cardiomyocyte-restricted loss of Fog2 recapitulated the cardiac and coronary defects of the Fog2 germline knockouts. Later cardiomyocyte-restricted loss of Fog2 (Fog2MC) did not result in defects in cardiac structure or coronary vessel formation. However, Fog2MC adult mice had severely depressed ventricular function and died at 8-14 weeks. Fog2MC adult hearts displayed a paucity of coronary vessels. This was associated with myocardial hypoxia, increased cardiomyocyte apoptosis, and cardiac fibrosis. Induced inactivation of Fog2 in adult heart resulted in similar phenotype, as did ablation of FOG2 interaction with the transcription factor GATA4. Loss of FOG2 or FOG2-GATA4 interaction altered expression of a panel of angiogenesis-related genes. Collectively, our data indicated that FOG2 regulates adult heart function and coronary angiogenesis.

 
Overall design Fog2flox/delta; MHCaCre (3) compared to Fog2flox/delta; no Cre (3). One heart per sample.
 
Contributor(s) Zhou B, Pu WT, Kong S
Citation(s) 19411759
Submission date Mar 03, 2009
Last update date Feb 11, 2019
Contact name Sek Won Kong
E-mail(s) [email protected]
Phone 617-919-2689
Organization name Boston Children's Hospital
Department Informatics Program
Lab EN137
Street address 300 Longwood Avenue
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (6)
GSM377182 Fog2flox/delta; no Cre biological replicate 1
GSM377183 Fog2flox/delta; no Cre biological replicate 2
GSM377184 Fog2flox/delta; no Cre biological replicate 3
Relations
BioProject PRJNA114775

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE15078_RAW.tar 21.1 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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