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Status |
Public on Sep 09, 2021 |
Title |
HD-ZIP IV transcription factor OCL4 and 21-nt phasiRNAs are dispensable for male fertility in maize anthers |
Organism |
Zea mays |
Experiment type |
Expression profiling by high throughput sequencing Non-coding RNA profiling by high throughput sequencing
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Summary |
The maize ocl4 mutants showed differing levels of male sterility, which seems correlating with the quantity of 21-nt phasiRNA remaining during pre-meiotic stage. In this case, sRNA and transcriptome was profiled and compared in this study, showed that OCL4 and 21-nt phasiRNAs are dispensable for male fertility in maize anthers.
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Overall design |
Examination of maize phasiRNAs in ocl4 mutants by high throughput sequencing for RNA-seq.
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Contributor(s) |
Yadava P, Tamim S, Zhang H, Teng C, Zhou X, Meyers BC, Walbot V |
Citation(s) |
33689028 |
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Submission date |
May 12, 2020 |
Last update date |
Sep 10, 2021 |
Contact name |
Blake C. Meyers |
E-mail(s) |
[email protected]
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Phone |
314-587-1422
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Organization name |
Donald Danforth Plant Science Center
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Lab |
Meyers lab
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Street address |
975 N Warson Road
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City |
St. Louis |
State/province |
MO |
ZIP/Postal code |
63132 |
Country |
USA |
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Platforms (1) |
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Samples (16)
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GSM4550675 |
ocl4 maize anthers, 0.4 to 0.7 mm, sterile phenotype (score = 0); sample ZOO 16 #1 (RNA-seq) |
GSM4550676 |
ocl4 maize anthers, 0.4 to 0.7 mm, sterile phenotype (score = 0); sample ZOO 19 #28 (RNA-seq) |
GSM4550677 |
ocl4 maize anthers, 0.4 to 0.7 mm, sterile phenotype (score = 0); sample ZOO 16 #6 (RNA-seq) |
GSM4550678 |
ocl4 maize anthers, 0.4 to 0.7 mm, sterile phenotype (score = 0); sample ZOO 19 #23 (RNA-seq) |
GSM4550679 |
ocl4 maize anthers, 0.4 to 0.7 mm, partial fertile phenotype (score = 1); sample ZOO 19#24 (RNA-seq) |
GSM4550680 |
ocl4 maize anthers, 0.4 to 0.7 mm, partial fertile phenotype (score = 1); sample ZOO 21 #35 (RNA-seq) |
GSM4550681 |
ocl4 maize anthers, 0.4 to 0.7 mm, fertile phenotype (score = 4); sample ZOO 21 #36 (RNA-seq) |
GSM4550682 |
ocl4 maize anthers, 0.4 to 0.7 mm, partial fertile phenotype (score = 2); sample ZOO 16#2 (RNA-seq) |
GSM4550683 |
ocl4 maize anthers, 0.4 to 0.7 mm, sterile phenotype (score = 0); sample ZOO 16#1 (small RNA-seq) |
GSM4550684 |
ocl4 maize anthers, 0.4 to 0.7 mm, sterile phenotype (score = 0); sample ZOO 19 #28 (small RNA-seq) |
GSM4550685 |
ocl4 maize anthers, 0.4 to 0.7 mm, sterile phenotype (score = 0); sample ZOO 16 #6 (small RNA-seq) |
GSM4550686 |
ocl4 maize anthers, 0.4 to 0.7 mm, sterile phenotype (score = 0); sample ZOO 19 #23 (small RNA-seq) |
GSM4550687 |
ocl4 maize anthers, 0.4 to 0.7 mm, partial fertile phenotype (score = 1); sample ZOO 19#24 (small RNA-seq) |
GSM4550688 |
ocl4 maize anthers, 0.4 to 0.7 mm, partial fertile phenotype (score = 2); sample ZOO 16#2 (small RNA-seq) |
GSM4550689 |
ocl4 maize anthers, 0.4 to 0.7 mm, partial fertile phenotype (score = 1); sample ZOO 21 #35 (small RNA-seq) |
GSM4550690 |
ocl4 maize anthers, 0.4 to 0.7 mm, fertile phenotype (score = 4); sample ZOO 21 #36 (small RNA-seq) |
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Relations |
BioProject |
PRJNA632352 |
SRA |
SRP261362 |
Supplementary file |
Size |
Download |
File type/resource |
GSE150446_RAW.tar |
1.8 Gb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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