|
| Status |
Public on Apr 02, 2020 |
| Title |
Expression profiling by high-throughput RNA-sequencing following PGC1β and ERRα knockdown in HCT116 cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal of this experiment was to determine differential gene regulation of PGC1𝛽 or ERR𝛼 compared to the control.
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| |
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| Overall design |
To identify transcripts that were specifically affected following PGC1β and ERRα knockdown. RNA was isolated using QIAGEN Rneasy kit. Six unstranded (poly A only) RNA samples were sequenced by the UNMC Genomics Core facility using the TrueSeq mRNA protcol.
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| |
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| Contributor(s) |
Frodyma DE, Berg J, Lewis RE, Fisher KW |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 01, 2020 |
| Last update date |
Apr 04, 2020 |
| Contact name |
Danielle Frodyma |
| Organization name |
University of Nebraska Medical Center
|
| Street address |
Buffet Cancer Center 9.12.379
|
| City |
Omaha |
| State/province |
NE |
| ZIP/Postal code |
68198 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA622524 |
| SRA |
SRP254908 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE147905_HCT116siCONT1_genes_fullname_rsem.xlsx |
6.1 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116siCONT1_isoforms_fullname_rsem.xlsx |
14.0 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116siCONT2_genes_fullname_rsem.xlsx |
6.1 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116siCONT2_isoforms_fullname_rsem.xlsx |
13.9 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116siERRa7_genes_fullname_rsem.xlsx |
6.1 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116siERRa7_isoforms_fullname_rsem.xlsx |
13.9 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116siERRa8_genes_fullname_rsem.xlsx |
6.1 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116siERRa8_isoforms_fullname_rsem.xlsx |
14.4 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116sipgc1b2_genes_fullname_rsem.xlsx |
6.1 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116sipgc1b2_isoforms_fullname_rsem.xlsx |
14.4 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116sipgc1b6_genes_fullname_rsem.xlsx |
6.1 Mb |
(ftp)(http) |
XLSX |
| GSE147905_HCT116sipgc1b6_isoforms_fullname_rsem.xlsx |
14.4 Mb |
(ftp)(http) |
XLSX |
| GSE147905_RAW.tar |
4.5 Mb |
(http)(custom) |
TAR (of TSV) |
| GSE147905_erra_rnaseq_differential_expression.xlsx |
3.5 Mb |
(ftp)(http) |
XLSX |
| GSE147905_pgc1b_rnaseq_differential_expression.xlsx |
3.5 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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