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| Status |
Public on Aug 03, 2020 |
| Title |
Two subsets of stem-like CD8+ memory T cell progenitors with distinct fate commitments in humans |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
T cell memory relies on the generation of antigen-specific progenitors with stem-like properties. However, the identity of these progenitors has remained unclear, precluding a full understanding of the differentiation trajectories that underpin the heterogeneity of memory and effector T cells. Here, we used a series of high-dimensional approaches guided by single-cell RNA sequencing data to map the organizational structure of the human CD8+ memory T cell pool under physiological conditions. We identified two previously unrecognized subsets of clonally, epigenetically, functionally, phenotypically, and transcriptionally distinct stem-like CD8+ memory T cells. Progenitors lacking the inhibitory receptors programmed death-1 (PD-1) and T cell immunoreceptor with Ig and ITIM domains (TIGIT) were committed to a functional lineage, whereas progenitors expressing PD-1 and TIGIT were committed to a dysfunctional, exhausted-like lineage. Collectively, these data revealed the existence of parallel differentiation programs in the human CD8+ memory T cell pool, with potentially broad implications for the development of immunotherapies and vaccines.
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| Overall design |
RNA-Seq profiling of TSTEM and TPEX sorted from four healthy donors' peripheral blood after in vitro stimulation.
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| Contributor(s) |
Galletti G, De Simone G, Mazza EM, Simone P, Peano C, Lugli E |
| Citation(s) |
33046887 |
| |
| Submission date |
Mar 23, 2020 |
| Last update date |
Feb 24, 2021 |
| Contact name |
Emilia Maria Cristina Mazza |
| E-mail(s) |
[email protected]
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| Organization name |
Humanitas Clinical and Research Center
|
| Street address |
Via Alessandro Manzoni, 56
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| City |
Rozzano, Milan |
| ZIP/Postal code |
20089 |
| Country |
Italy |
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| Platforms (1) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE147398 |
Two subsets of stem-like CD8+ memory T cell progenitors with distinct fate commitments in humans |
|
| Relations |
| BioProject |
PRJNA614484 |
| SRA |
SRP253704 |
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