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| Status |
Public on May 21, 2020 |
| Title |
Transcriptional profiling of the probiotic E. coli Nissle 1917 strain under simulated microgravity and normal gravity |
| Organism |
Escherichia coli |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We performed comparative transcriptomic profiling of E. coli Nissle 1917 (EcN) to determine the effect of microgravity (MG) on cell growth and metabolism.
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| Overall design |
Growth phase: Exponential and stationary growth phase.
Culture condition: MG and normal gravity (NG) condition, in duplicate, M9 minimal media with glucose, 37°C, total 8 samples
MG condition can be simulated by rotary cell culture system (RCCS) from Synthecon Inc. (Houston, TX, USA) when it is rotated around the horizontal axis. On the other hand, NG condition can be simulated by RCCS when it is rotated around the vertical axis.
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| Contributor(s) |
Yim J, Cho SW, Kim B, Park S, Han YH, Seo SW |
| Citation(s) |
32290466 |
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| Submission date |
Mar 20, 2020 |
| Last update date |
May 21, 2020 |
| Contact name |
Sang Woo Seo |
| Organization name |
Seoul National University
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| Street address |
1, Gwanak-ro, Gwanak-gu
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| City |
Seoul |
| ZIP/Postal code |
08826 |
| Country |
South Korea |
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| Platforms (1) |
| GPL21433 |
Illumina HiSeq 4000 (Escherichia coli) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA613653 |
| SRA |
SRP253467 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE147272_RAW.tar |
2.1 Mb |
(http)(custom) |
TAR (of GTF) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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