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| Status |
Public on Aug 26, 2021 |
| Title |
Characterization of pancreatic progenitor differentiation with GiBi-generated DE cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report the GiBi protocol to generate functional DE cells that can further enter pancreatic lineages. To understand the transcriptome dynamics during the generation of these pancreatic progenitor cells, we harvested cells at different time points of the differentiation for RNA sequencing. We find that during this process, genes that are related to pluripotency maintenance were decreasing and gastrulation-related genes were transiently expressed in the middle. More importantly, genes that are critical in pancreatic specification got significantly upregulated across the differentiation.
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| Overall design |
Every other day from D0 to D14 of the pancreatic progenitor differentiation, two biological replicates for each time point, we harvested in total 14 samples for RNA sequencing.
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| Contributor(s) |
Jiang Y, Lian X |
| Citation(s) |
34450037 |
| |
| Submission date |
Mar 14, 2020 |
| Last update date |
Oct 06, 2021 |
| Contact name |
Yuqian Jiang |
| E-mail(s) |
[email protected]
|
| Organization name |
PSU
|
| Street address |
W345 millennium science complex
|
| City |
University park |
| State/province |
PA |
| ZIP/Postal code |
16801 |
| Country |
USA |
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| Platforms (1) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA612623 |
| SRA |
SRP252813 |
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