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| Status |
Public on Oct 12, 2009 |
| Title |
Immunopurified mRNA-ribosome complexes expose cell-type specific plasticity in response to hypoxia in Arabidopsis |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by array
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| Summary |
Plant organs are comprised of distinct cell types with unique assemblages of mRNAs. This is a collection of CEL files of mRNA profiles of the total steady-state mRNAs and polysomal mRNAs of distinct cell types of the whole root and shoot of 7-d-old Arabidopsis thaliana seedlings. The cell type specific mRNA populations are those present in ribosome-mRNA complexes. This sub-population of mRNAs was obtained by first establishing a collection of Arabidopsis lines that express a FLAG-epitope tagged ribosomal protein L18 (RPL18) directed by promoters expressed in specific cell types and regions. Thirteen different promoter:FLAG-RPL18 lines were used. The targeted cell types and promoters included root atrichoblast (non-hair) epidermal cells (pGL2), root endodermis (pSCR), root stelar xylem and pericycle (pWOL, pSHR), root phloem companion cells (phloem CC) (pSUC2, pSultr2;2), root proliferating cells (pRPL11C), root cortex meristematic cells (pCO2), root cortex elongation/maturation cells (pPEP), shoot mesophyll (pRBCS), shoot epidermis (pCER5), shoot guard cells (pKAT1), shoot bundle sheath (pSultr2;2), shoot phloem CC (pSUC2) and shoot trichomes (pGL2). A CaMV 35S promoter:FLAG-RPL18 line was used to obtain the polysomal mRNA of multiple cell types. The immunopurification of ribosome-mRNA complexes of specific cell types/regions was accomplished by the method described in Zanetti et al. (Plant Physiology, 138, 624-635; 2005). Hybridization of the immunopurified mRNAs to the Affymetrix ATH1 DNA microarray platform and subsequent data analysis permitted the identification of transcripts that are enriched or depleted in specific cell types/regions of roots and shoots. The dataset includes samples from cell types/regions from seedlings grown under control conditions and cell types/regions of seedlings exposed to low oxygen stress (hypoxia) for 2 h.
Keywords: cell-type specific expression, hypoxic stress, polysomal mRNA, abiotic stress, atrichoblasts, epidermis, cortex, endodermis, stele, phloem companion cells, guard cells, mesophyll
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| Overall design |
79 samples, 2 conditions (2 h hypoxia stress, 2 h non-stress), 2 RNA pools (Total mRNA and polysomal mRNA), 2 organs, 13 promoter lines, 2-4 replicates
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| Contributor(s) |
Mustroph A, Bailey-Serres J |
| Citation(s) |
19843695 |
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| Submission date |
Jan 21, 2009 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Angelika Mustroph |
| E-mail(s) |
[email protected]
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| Organization name |
University Bayreuth
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| Department |
Plant Physiology
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| Lab |
Angelika Mustroph
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| Street address |
Universitaetsstr. 30, NWI
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| City |
Bayreuth |
| ZIP/Postal code |
95440 |
| Country |
Germany |
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| Platforms (1) |
| GPL198 |
[ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array |
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| Samples (79)
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| This SubSeries is part of SuperSeries: |
| GSE14578 |
Immunopurified mRNA-ribosome complexes expose cell-type specific plasticity during hypoxia in Arabidopsis |
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| Relations |
| Affiliated with |
GSE69995 |
| BioProject |
PRJNA114489 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE14502_RAW.tar |
182.8 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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