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| Status |
Public on Jan 28, 2020 |
| Title |
Transcriptomics analysis of lineage-restricted Dendritic-cell (DC) progenitor cells in obesity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The overall goal of the project is to understand transcript-level changes in DC-progenitors from bone marrow of diet-induced obese mice by Next Generation Sequencing (NGS; RNASeq)
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| Overall design |
DC-restricted progenitors (Lin-/c-kit(int)/Sca1(lo)/CD115+/Flt3+; ~50,000 cells) from bone marrow (BM) compartment of high fat (60kcal % fat) & control chow (10 kcal%fat)-fed mic (n=4 per group) were flow-sorted and RNA was isolated.
The whole transcriptome was amplified and library was constructed using NuGen Ovation Solo RNASeq System; and evaluated on high-sensitivity DNAchip (Agilent) and sequenced on a HiSeq 3000 platform
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| Contributor(s) |
Pirooznia M, Mishra A, Levine S |
| Citation(s) |
32184787 |
| |
| Submission date |
Jan 27, 2020 |
| Last update date |
Mar 23, 2020 |
| Contact name |
Mehdi Pirooznia |
| E-mail(s) |
[email protected]
|
| Phone |
410-340-6052
|
| Organization name |
National Institutes of Health
|
| Street address |
12 SOUTH DR
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA603380 |
| SRA |
SRP245437 |
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