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| Status |
Public on Jan 03, 2020 |
| Title |
microRNA expression analysis in fly ovaries from wuho and mei-p26 mutants |
| Organism |
Drosophila melanogaster |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Purpose: The goal of this study is to analyze microRNA affected by wuho and mei-p26 mutations in Drosophila ovary
Ovarian microRNA prolife of 7 day old control flies and flies bearing wuho and mei-p26 mutations, in duplicate.Sample libraries were prepared using the QIAseq miRNA Library Kit (QIAGEN) according to the manufacturer's protocols. Adaptors are ligated sequentially to the 3' and 5' ends of miRNAs. Subsequently, universal cDNA synthesis with UMI assignment, cDNA cleanup, library amplification and library cleanup are performed. Libraries were sequenced on an Illumina instrument (75-cycle single-end read, 75SE). Sequencing data was processed using the Illumina software BCL2FASTQ v2.20.
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| Overall design |
Ovarian microRNA prolife of 7 day-old adult control flies and flies bearing wuho and mei-p26 mutanst in duplicate using Illumina instrument (75-cycle single-end read, 75SE).
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| Contributor(s) |
Hsu H, Rastegari E |
| Citation(s) |
31941704 |
| |
| Submission date |
Jan 02, 2020 |
| Last update date |
Apr 06, 2020 |
| Contact name |
Hweijan Hsu |
| E-mail(s) |
[email protected]
|
| Phone |
886-2-2787-1541
|
| Organization name |
Academia Research
|
| Department |
Institute of Cellular Biology
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| Lab |
Room 337
|
| Street address |
no 128, Sec 2, Academia Rd. Nankang
|
| City |
Taipei |
| ZIP/Postal code |
11529 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA598570 |
| SRA |
SRP239274 |
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