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| Status |
Public on Dec 27, 2020 |
| Title |
Divergent role for STAT5 in the adaptive responses of natural killer cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Natural killer (NK) cells are innate lymphocytes with the capacity to elicit adaptive features, including clonal expansion and immunological memory. Because signal transducer and activator of transcription 5 (STAT5) is essential for NK cell development, the role of this transcription factor and its upstream cytokines IL-2 and IL-15 during infection have not been carefully investigated. In this study, we investigate how STAT5 regulates transcription during viral infection. We demonstrate that STAT5 is induced in NK cells by IL-12 and STAT4 early after infection, and that partial STAT5 deficiency results in a defective capacity of NK cells to generate long-lived memory cells. Furthermore, we find a functional dichotomy of IL-2 and IL-15 signaling outputs during viral infection, whereby both cytokines drive clonal expansion, but only IL-15 is required for memory NK cell survival. We thus highlight a novel role for STAT5 signaling in promoting an optimal antiviral NK cell response.
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| Overall design |
RNA-seq was performed on Ly49H+ NK cells harvested during in vivo MCMV infection.
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| Contributor(s) |
Lau CM, Sun JC |
| Citation(s) |
33326784 |
| |
| Submission date |
Jan 02, 2020 |
| Last update date |
Dec 27, 2020 |
| Contact name |
Colleen Lau |
| Organization name |
Cornell University
|
| Department |
Microbiology & Immunology
|
| Street address |
930 Campus Road
|
| City |
Ithaca |
| State/province |
New York |
| ZIP/Postal code |
14850 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA598546 |
| SRA |
SRP239264 |
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