 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Dec 31, 2022 |
| Title |
Role of high fat diet on silica-induced lung toxicity in rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
From an occupational standpoint, exposure to silica can have devastating consequences. An estimated 2.3 million workers in the U.S. are exposed to dust containing crystalline silica, annually. In addition, of the 140 million people over the age of 20, employed in the U.S., 30% are obese. If and how diet-induced obesity modifies silica-induced pulmonary toxicity is unknown. Therefore, the objective of this study was to determine the effect of diet-induced obesity, if any, on silica induced pulmonary toxicity. Rats (Fischer 344, male) were fed either a regular-fat diet (RFD; 18% kcal as fat) (Envigo, Indianapolis, IN) or a high-fat diet (HFD; 60% kcal as fat) (Envigo, Indianapolis, IN) and exposed by whole-body inhalation to either air or crystalline silica (15 mg/m3, 6 hours/day, 5 days). At designated post-exposure time intervals (1, 3, 6, and 9 months), pulmonary toxicity was determined. Silica inhalation resulted in pulmonary toxicity, which progressed across all post-exposure time points, as evidenced by enhanced neutrophil infiltration, increased LDH levels, enhanced oxidant production, and increased inflammatory cytokine levels. The incidence and severity of silica-induced lung pathology was similar between the two diet groups up to 6 months post-exposure. However, by 9 months post-exposure, silica-induced pathology tended to be slightly more severe in animals fed an RFD compared to those fed an HFD. Lung gene expression profiles were then determined in the rats euthanized at the 3- and 9-month post-silica exposure time intervals by RNA sequencing to identify transcript differences between the two timepoints.
|
| |
|
| Overall design |
Lung RNA profiles of rats exposed to air, crystalline silica (full body inhalation exposure), a high fat diet, or a high fat diet and crystalline silica were generated by deep sequencing, 4 ≤ n ≤ 6, using Illumina HiSeq.
|
| |
|
| Contributor(s) |
Sager TM, Umbright CM, Joseph P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Dec 20, 2019 |
| Last update date |
Dec 31, 2022 |
| Contact name |
Christina M Umbright |
| Organization name |
DHHS/PHS/CDC/NIOSH
|
| Department |
HELD
|
| Lab |
L-4324
|
| Street address |
1095 Willowdale Road
|
| City |
Morgantown |
| State/province |
WV |
| ZIP/Postal code |
26505 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (42)
|
|
| Relations |
| BioProject |
PRJNA597033 |
| SRA |
SRP238405 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE142446_SO03Lu_tophat_counts.csv.gz |
548.7 Kb |
(ftp)(http) |
CSV |
| GSE142446_SO09Lu_tophat_counts.csv.gz |
659.8 Kb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...