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| Status |
Public on Dec 14, 2019 |
| Title |
Escalation in the host-pathogen arms race: a host resistance response corresponds to a heightened bacterial virulence response |
| Organisms |
Sorghum bicolor; Xanthomonas vasicola |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The zig-zag model of host-pathogen interaction describes the relative strength of defense response across a spectrum of pathogen-induced plant phenotypes. A stronger defense response results in increased resistance. Here, we investigate the strength of pathogen virulence during disease and place these findings in the context of the zig-zag model. Xanthomonas vasicola pv. holcicola (Xvh) causes sorghum bacterial leaf streak. Despite being widespread, this disease has not been described in detail at the molecular level. We divided diverse sorghum genotypes into three groups based on disease symptoms: water-soaked lesions, red lesions, and resistance. Bacterial growth assays confirmed that these three phenotypes represent a range of resistance and susceptibility. To simultaneously reveal defense and virulence responses across the spectrum of disease phenotypes, we performed dual RNA-seq on Xvh-infected sorghum. Consistent with the zig-zag model, the expression of plant defense-related genes was strongest in the resistance interaction. Surprisingly, bacterial virulence genes related to the type III secretion system (T3SS) and type III effectors (T3Es) were also most highly expressed in the resistance interaction. This expression pattern was observed at multiple time points within the sorghum-Xvh pathosystem. Further, a similar expression pattern was observed in Arabidopsis infected with Pseudomonas syringae for effector-triggered immunity via AvrRps4 but not AvrRpt2. Specific metabolites were able to repress the Xvh virulence response in vitro and in planta suggesting a possible signaling mechanism. Taken together, these findings reveal multiple permutations of the continually evolving host-pathogen arms race from the perspective of host defense and pathogen virulence responses.
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| Overall design |
Dual RNA-seq profiled in 7 combinations (21 samples) of Xanthomonas vasicola pv. holcicola (Xvh) and three sorghum genotypes. The fourth leaf of 14-day-old sorghum plants was syringe-inoculated with bacterial suspensions and a mock treatment. At 48 h after inoculation, the infected tissue areas in leaves were harvested. Total RNA was extracted, followed by ribosomal RNA depletion, cDNA library preparation, and RNA-sequencing. Bacteria were grown on plates containing NYGA medium were also analyzed. For the samples, three independent replicates were taken.
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| Web link |
https://doi.org/10.1371/journal.ppat.1009175
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| Contributor(s) |
Wang Q, Shakoor N, Boyher A, Veley KM, Berry JC, Mockler TC, Bart RS |
| Citation(s) |
33428681 |
| |
| Submission date |
Dec 13, 2019 |
| Last update date |
Feb 01, 2021 |
| Contact name |
Rebecca Bart |
| E-mail(s) |
[email protected]
|
| Phone |
314-587-1696
|
| Organization name |
Donald Danforth Plant Science Center
|
| Lab |
Bart Lab
|
| Street address |
975 North Warson Road
|
| City |
Saint Louis |
| State/province |
MO |
| ZIP/Postal code |
63132 |
| Country |
USA |
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| Platforms (3) |
| GPL27912 |
Illumina HiSeq 4000 (Xanthomonas vasicola) |
| GPL27913 |
Illumina HiSeq 4000 (Sorghum bicolor) |
| GPL27914 |
Illumina HiSeq 4000 (Sorghum bicolor; Xanthomonas vasicola) |
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| Samples (21)
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| Relations |
| BioProject |
PRJNA595608 |
| SRA |
SRP237552 |
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