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| Status |
Public on May 05, 2020 |
| Title |
Cell Specific Transcriptional Responses to Heat Shock in the Mouse Utricle Epithelium |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To determine the transcriptional responses to heat shock of inner ear sensory hair cells and supporting cells, we performed cell-type-specific transcriptional profiling using the RiboTag method, which allows for immunoprecipitation of actively translating mRNAs from specific cell types. RNA-Seq differential gene expression analyses demonstrated that RiboTag identified known cell type-specific markers as well as new markers for hair cells and supporting cells. Gene expression differences suggest that both hair cells and supporting cells exhibit a transcriptional heat shock response. However, hair cells and supporting cells expressed different members of the heat shock protein family in response to heat stress, and supporting cells expressed a larger number of HSPs. Only one HSP, Chaperonin Containing T-Complex Polypeptide 1 Subunit 8, (CCT8) was uniquely induced in hair cells. Together our data indicate that hair cells exhibit a limited but unique heat shock response, and supporting cells exhibit a broader and more robust transcriptional response to protective heat stress.
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| Overall design |
RNA-seq comparisons of control (CON) versus Heat-shock treated cultured utricles. The RNA was isolated fom ribotag-enriched immunoprecipates (IP) and compared to RNA isolated from input to identify genes enriched in the IP. Two cre-expressing mouse lines were used to obtain either hair-cell enriched IP samples (Gfi1-Cre) or supporting cell enrich IP samples (GLAST-CreER).
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| Contributor(s) |
Sadler E, Ryals MR, May LA, Martin D, Welsh N, Boger ET, Morell RJ, Hertzano RP, Cunningham LL |
| Citation(s) |
32528249 |
| |
| Submission date |
Oct 30, 2019 |
| Last update date |
Nov 21, 2022 |
| Contact name |
Robert Morell |
| E-mail(s) |
[email protected]
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| Organization name |
National Institute on Deafness and Other Communication Disorders
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| Street address |
35A Convent Drive, Room 1F-103
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL18480 |
Illumina HiSeq 1500 (Mus musculus) |
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| Samples (34)
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| Relations |
| BioProject |
PRJNA580444 |
| SRA |
SRP227469 |
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