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| Status |
Public on Jul 13, 2022 |
| Title |
10x single cell sequencing for primary neurons transcriptomes after sevoflurane exposure |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We thus isolated cortex from mice, with or without sevoflurane exposure, and performed single cell sequencing. Single cell sequencing is a innovative experimental procedures which allows the identification of various subsets of cells in the neverous system after sevoflurane exposure. Isolated cells were concentrated to 1000 cells/uL and approximately 17,000 cells were loaded into each channel to generate single-cell Gel Bead-In-Emulsions (GEMs), which led to expected mRNA barcoding of 10,000 single-cells for each sample. Seurat package was used for cell normalization and regression. Sevoflurane and control groups were aligned and combined using Seurat. Using t-distributed stochastic neighbor embedding (tSNE), 13 cluters of cells were visualized. Cells were assigned to various clusters based on strong expression of specific genes.
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| Overall design |
Examination of 2 different exposure in primary neurons
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| |
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| Contributor(s) |
Zhang L |
| Citation(s) |
33928466 |
| |
| Submission date |
Oct 17, 2019 |
| Last update date |
Jul 13, 2022 |
| Contact name |
yanyong cheng |
| E-mail(s) |
[email protected]
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| Organization name |
Shanghai Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine
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| Department |
Department of Anesthesiology
|
| Street address |
639 Zhizaoju Road
|
| City |
shanghai |
| ZIP/Postal code |
200011 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA578089 |
| SRA |
SRP226037 |
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