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Series GSE138342 Query DataSets for GSE138342
Status Public on Oct 03, 2019
Title Epigenetic pattern after EZH1,2 inhibition in lymohoma cells
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary Although global H3K27me3 reprogramming is a hallmark of cancer, no effective therapeutic strategy for H3K27me3-high malignancies harboring EZH2WT/WT has yet been established. We explored epigenome and transcriptome in EZH2WT/WT aggressive lymphomas, and found that mutual interference and compensatory function of co-expressed EZH1 and EZH2 rearrange their own genome-wide distribution, thereby establishing restricted chromatin and gene expression signatures. Direct comparison of leading compounds introduced potency and a mechanism-of-action of the EZH1/2 dual-inhibitor (valemetostat). The synthetic lethality was observed in all lymphoma models and primary adult T-cell leukemia–lymphoma (ATL) cells. Opposing actions of EZH1/2-polycomb and SWI/SNF complexes are required for facultative heterochromatin formation. Inactivation of chromatin-associated genes (ARID1A, SMARCA4/BRG1, SMARCB1/SNF5, KDM6A/UTX, BAP1, KMT2D/MLL2) and oncovirus infection (HTLV-1, EBV) trigger EZH1/2 perturbation and H3K27me3 deposition. Our study provides the mechanism-based rationale for chemical dual-targeting of EZH1/2 in cancer epigenome.
 
Overall design We explored epigenome (EZH1/2 occupancies and histone modifications) in aggressive lymphoma samples and cell lines treated with EZH1/2 inhibitors by using ChIP-on-chip platform
 
Contributor(s) Yamagishi M, Hori M, Fujikawa D
Citation(s) 31747604
Submission date Oct 02, 2019
Last update date Nov 27, 2019
Contact name Makoto Yamagishi
E-mail(s) [email protected]
Organization name The University of Tokyo
Lab Lab. Tumor Cell Biology
Street address 4-6-1, Shirokanedai
City Minato-ku
State/province Tokyo
ZIP/Postal code 108-8639
Country Japan
 
Platforms (1)
GPL19784 Agilent-027954 SurePrint G3 Human Promoter Microarray 2x400K [Probe Name version]
Samples (117)
GSM4105705 Normal1_controlIgG
GSM4105706 Normal1_EZH1
GSM4105707 Normal2_2_controlIgG
Relations
BioProject PRJNA575541

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE138342_Matrix_data_10_DLCL2_EZHi_H3K27me3_Coc.xlsx 22.3 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_11_DLCL2_EZHi_EZH1_Coc.xlsx 18.7 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_12_HUT102_OR-S1_H3K27me3_Coc.xlsx 16.9 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_13_HUT102_OR-S1_EZH1_Coc.xlsx 16.7 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_14_HUT102_OR-S1_EZH2_Coc.xlsx 18.3 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_15_TL-Om1_OR-S1_RelA_Coc.xlsx 14.0 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_16_TL-Om1_OR-S1_RelB_Coc.xlsx 13.9 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_17_TL-Om1_OR-S1_H2AK119ub_Coc.xlsx 18.0 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_18_TL-Om1_OR-S1_RNAPolII_Coc.xlsx 18.3 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_19_TL-Om1_OR-S1_H3K27ac_Coc.xlsx 18.7 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_1_Normal__ATL_EZH1_Coc.xlsx 37.6 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_20_TL-Om1_shEZH1_2_H3K27me3_Coc.xlsx 20.3 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_21_TL-Om1_shEZH1_2_SUZ12_Coc.xlsx 22.6 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_22_MCF7_EZH1_2_OE_FLAG_Coc.xlsx 20.7 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_23_MCF7_EZH1_2_OE_EZH1_Coc.xlsx 20.8 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_24_SUDHL8_OR-S1_H3K27me3_Coc.xlsx 18.6 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_25_SUDHL8_OR-S1_EZH1_Coc.xlsx 18.4 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_26_SUDHL8_OR-S1_EZH2_Coc.xlsx 18.1 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_27_TL-Om1_EZHi_ARID1A_Coc.xlsx 20.6 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_28_TL-Om1_EZHi_BRG1_Coc.xlsx 20.5 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_29_LCL_shMLL2_EZH1_Coc.xlsx 18.2 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_2_Normal__ATL_EZH2_Coc.xlsx 58.4 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_30_LCL_shMLL2_EZH2_Coc.xlsx 18.3 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_31_LCL_shMLL2_H3K27me3_Coc.xlsx 18.4 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_32_LCL_shMLL2_H3K4me3_Coc.xlsx 17.1 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_33_MCF7_shBAF_H3K27me3_Coc.xlsx 23.9 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_34_MCF7_shBAF_EZH1_Coc.xlsx 23.4 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_35_MCF7_shBAF_EZH2_Coc.xlsx 23.5 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_3_TL-Om1_EZHi_EZH1_Coc.xlsx 21.5 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_4_TL-Om1_EZHi_EZH2_Coc.xlsx 20.9 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_5_TL-Om1_EZHi_YY1_Coc.xlsx 21.5 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_6_TL-Om1_EZHi_H3K4me3_Coc.xlsx 21.5 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_7_TL-Om1_shEZH1_2_EZH1_Coc.xlsx 20.8 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_8_TL-Om1_shEZH1_2_EZH2_Coc.xlsx 21.7 Mb (ftp)(http) XLSX
GSE138342_Matrix_data_9_TL-Om1_shYY1_GSK126_EZH1_Coc.xlsx 20.3 Mb (ftp)(http) XLSX
GSE138342_RAW.tar 5.0 Gb (http)(custom) TAR (of TXT)
Processed data are available on Series record
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