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Series GSE137680 Query DataSets for GSE137680
Status Public on Aug 01, 2020
Title Gene expression analysis of PBMC's from IBD subjects stimulated by combinations of LPS, MDP, anti-CD3/anti-CD28 antibodies, and anti-IL10R antibodies.
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We systematically investigated the transcriptomic response pattern of PBMC from 41 patients with IBD to characterize relevant stimuli that target different aspects of innate and adaptive immune cell responses. Lipopolysaccharide(LPS), muramyl-dipeptide (L18MDP), T cell receptor and co-stimulation (αCD3/αCD28 coated beads), and IL-10 signalling blockade were used to sitmulate subject PBMC's alone or in combination based on the concept that innate pathogen recognition receptor responses (in particular NOD2 and TLR4), T cell responses, and IL-10 signalling defects are implicated by multiple genetic IBD susceptibility loci and Mendelian forms of IBD.
 
Overall design PBMC's isolated from IBD patients were subjected to microarray gene expression analysis after 16 hours of stimulation (by LPS, L18MDP and anti-CD3/anti-CD28 coated beads) in the presence or absence of IL-10 receptor-blocking antibodies.
 
Contributor(s) Aschenbrenner D, Quaranta M, Steere B, Rocha G, Uhlig HH
Citation(s) 33037057
Submission date Sep 18, 2019
Last update date Nov 01, 2020
Contact name Boyd Steere
E-mail(s) [email protected]
Organization name Lilly
Department Immunology
Street address Lilly Corporate Center
City Indianapolis
State/province IN
ZIP/Postal code 46285
Country USA
 
Platforms (2)
GPL17585 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [probe set (exon) version]
GPL17586 [HTA-2_0] Affymetrix Human Transcriptome Array 2.0 [transcript (gene) version]
Samples (886)
GSM4084108 [gene] aCD3-aCD28 diagnosis-CD subject-129 biological rep-1
GSM4084109 [gene] aCD3-aCD28 diagnosis-CD subject-138 biological rep-2
GSM4084110 [gene] aCD3-aCD28 diagnosis-CD subject-186 biological rep-3
This SubSeries is part of SuperSeries:
GSE138009 Systems-level analysis of monocyte responses in inflammatory bowel disease identifies IL-10 and IL-1 cytokine networks that regulate IL-23
Relations
BioProject PRJNA574128

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE137680_RAW.tar 19.6 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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