|
| Status |
Public on Jun 15, 2021 |
| Title |
Gene Expression Changes during Chronic Social Isolation in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To investigate gene expression changes in Drosophila head tissues during social isolation, we performed RNA-sequencing on fruit fly head samples obtained from male flies that have been group-reared for 7 days (Grp), isolated (single-housed) for 7 days (Iso7) and isolated (single-housed) for only 1 day (Iso1). Using differential gene expression analysis, we found a group of candidate genes that are specific to chronic social isolation: they exhibited significant gene expression change in both comparisons of “Grp vs Iso1” and “Iso1 vs Iso7”.
|
| |
|
| Overall design |
Heads of wild type flies with group/isolation experience were collected at ZT0.5-2. Flies of three treatment conditions were collected: socially enriched animals (group reared for 7 days, “Grp”), chronic isolation animals (isolated for 7 days, “Iso7”) and acute isolation animals (isolated for 1 day, “Iso1”). For each condition, 3 replicated samples were collected and each sample contains 200 fly heads.
|
| |
|
| Contributor(s) |
Li W, Young MW |
| Citation(s) |
34408325 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R37 NS053087 |
Interdisciplinary Studies of Sleep and Circadian Rhythms |
THE ROCKEFELLER UNIVERSITY |
Michael W Young |
| R35 GM136237 |
Molecular pathways connecting sleep, stress, metabolism and longevity |
THE ROCKEFELLER UNIVERSITY |
Michael W Young |
|
| |
| Submission date |
Sep 16, 2019 |
| Last update date |
Sep 14, 2021 |
| Contact name |
Wanhe Li |
| E-mail(s) |
[email protected]
|
| Organization name |
The Rockefeller University
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL19132 |
Illumina NextSeq 500 (Drosophila melanogaster) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA565734 |
| SRA |
SRP221747 |
Less...
More...