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| Status |
Public on Jul 02, 2020 |
| Title |
Bimodal Adenine and Cytosine CRISPR Base Editing on DNA |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Adenine and cytosine base editors (ABEs and CBEs) represent a new genome editing technology that allows the programmable installation of A-to-G or C-to-T alterations on DNA. We engineered Streptococcus pyogenes Cas9-based adenine and cytosine base editor (SpACE) that enables efficient simultaneous introduction of A-to-G and C-to-T substitutions in the same base editing window on DNA.
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| Overall design |
HEK293T cells were transfected with a variety of base editor constructs. nCas9 and GFP expressing cells were negative controls. ABEmax and miniABEmax-V82G served as a control for A-to-G base editing on DNA and A-to-I editing on RNA. Target-AID served as a control for C-to-T base editing on DNA and C-to-U editing on RNA. All base editor constructs co-translationally expressed GFP (P2A-EGFP).The Cells were sorted for GFP based on FITC signal. RNA-seq was performed to measure transcriptional changes associated with different constructs.
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| Contributor(s) |
Grunewald J, Lareau C |
| Citation(s) |
32483364 |
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| Submission date |
Sep 13, 2019 |
| Last update date |
Oct 01, 2020 |
| Contact name |
Caleb Lareau |
| E-mail(s) |
[email protected]
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| Organization name |
Memorial Sloan Kettering
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| Street address |
417 E 68th St, Zuckerman - ZRC 1132
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
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| Samples (33)
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| GSM4078741 |
HEK293T cells, Target-AID_HEKsite2_Rep1 |
| GSM4078742 |
HEK293T cells, SpACE_HEKsite2_Rep1 |
| GSM4078743 |
HEK293T cells, GFP_Rep1 |
| GSM4078744 |
HEK293T cells, nCas9_HEKsite2_Rep2 |
| GSM4078745 |
HEK293T cells, ABEmax_HEKsite2_Rep2 |
| GSM4078746 |
HEK293T cells, miniABEmax-V82G_HEKsite2_Rep2 |
| GSM4078747 |
HEK293T cells, Target-AID_HEKsite2_Rep2 |
| GSM4078748 |
HEK293T cells, SpACE_HEKsite2_Rep2 |
| GSM4078749 |
HEK293T cells, GFP_Rep2 |
| GSM4078750 |
HEK293T cells, nCas9_HEKsite2_Rep3 |
| GSM4078751 |
HEK293T cells, ABEmax_HEKsite2_Rep3 |
| GSM4078752 |
HEK293T cells, miniABEmax-V82G_HEKsite2_Rep3 |
| GSM4078753 |
HEK293T cells, Target-AID_HEKsite2_Rep3 |
| GSM4078754 |
HEK293T cells, SpACE_HEKsite2_Rep3 |
| GSM4078755 |
HEK293T cells, GFP_Rep3 |
| GSM4078756 |
HEK293T cells, nCas9_RNF2_Rep1 |
| GSM4078757 |
HEK293T cells, ABEmax_RNF2_Rep1 |
| GSM4078758 |
HEK293T cells, miniABEmax-V82G_RNF2_Rep1 |
| GSM4078759 |
HEK293T cells, Target-AID_RNF2_Rep1 |
| GSM4078760 |
HEK293T cells, SpACE_RNF2_Rep1 |
| GSM4078761 |
HEK293T cells, nCas9_RNF2_Rep2 |
| GSM4078762 |
HEK293T cells, ABEmax_RNF2_Rep2 |
| GSM4078763 |
HEK293T cells, miniABEmax-V82G_RNF2_Rep2 |
| GSM4078764 |
HEK293T cells, Target-AID_RNF2_Rep2 |
| GSM4078765 |
HEK293T cells, SpACE_RNF2_Rep2 |
| GSM4078766 |
HEK293T cells, nCas9_RNF2_Rep3 |
| GSM4078767 |
HEK293T cells, ABEmax_RNF2_Rep3 |
| GSM4078768 |
HEK293T cells, miniABEmax-V82G_RNF2_Rep3 |
| GSM4078769 |
HEK293T cells, Target-AID_RNF2_Rep3 |
| GSM4078770 |
HEK293T cells, SpACE_RNF2_Rep3 |
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| Relations |
| BioProject |
PRJNA565401 |
| SRA |
SRP221588 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE137411_SpACE_allCounts.tsv.gz |
1011.5 Kb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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