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| Status |
Public on May 13, 2004 |
| Title |
Pig peri-implantation embryo development transcriptional profiling |
| Organism |
Sus scrofa |
| Experiment type |
Expression profiling by array
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| Summary |
A novel porcine cDNA microarray, containing PCR products selected for embryonic expression, was used for transcriptional profiling during conceptus development. Total RNA from quadruplicate samples of small spherical (1-5mm), large spherical (6-11mm), tubular (13-45mm), and filamentous (>100mm) was extracted, amplified, and converted to 32P labeled target and hybridized to membranes. Porcine peri-implantation embryo development order is from small spherical to large spherical, then to Tubular, then to filamentous. All this morphological transmission is within two days (day 10 to day 12). In this study, we investigated the gene expression changes by a specifically selected porcine embryo gene array. A number of genes were found to be differentially expressed during those developmental stages and confirmed by quantitative PCR.
Keywords = Gene expression
Keywords = porcine conceptus
Keywords = cDNA microarray
Keywords = peri-implantation
Keywords = transcriptional profiling
Keywords: time-course
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| Contributor(s) |
Lee SH, Zhao SH, Recknor JC, Nettleton D, Kang SK, Lee BC, Hwang WS, Tuggle CK |
| Citation(s) |
15791594 |
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| Submission date |
May 06, 2004 |
| Last update date |
Mar 15, 2012 |
| Contact name |
Christopher K Tuggle |
| E-mail(s) |
[email protected]
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| Phone |
515-294-4252
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| Fax |
515-294-2401
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| URL |
http://www.public.iastate.edu/~ans/faculty/tuggle.html
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| Organization name |
Iowa State University
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| Department |
Animal Science
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| Lab |
Molecular Genetics
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| Street address |
2255 Kildee Hall
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| City |
Ames |
| State/province |
IA |
| ZIP/Postal code |
50011 |
| Country |
USA |
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| Platforms (1) |
| GPL1209 |
Porcine 1000 embryo gene array |
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| Samples (16)
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| GSM22275 |
pig embryo array Small Spherical 1 |
| GSM22278 |
Pig embryo array Small Spherical sample 2 |
| GSM22281 |
Pig embryo array Small Spherical sample 3 |
| GSM22282 |
Pig embryo array Small Spherical sample 4 |
| GSM22283 |
Pig embryo array Large Spherical sample 1 |
| GSM22284 |
Pig embryo array Large Spherical sample 2 |
| GSM22285 |
Pig embryo array Large Spherical sample 3 |
| GSM22286 |
Pig embryo array Large Spherical sample 4 |
| GSM22287 |
Pig embryo array Tubular sample 1 |
| GSM22288 |
Pig embryo array Tubular sample 2 |
| GSM22289 |
Pig embryo array Tubular sample 3 |
| GSM22290 |
Pig embryo array Tubular sample 4 |
| GSM22291 |
Pig embryo array Filamentous sample 1 |
| GSM22292 |
Pig embryo array Filamentous sample 2 |
| GSM22293 |
Pig embryo array Filamentous sample 3 |
| GSM22294 |
Pig embryo array Filamentous sample 4 |
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| Relations |
| BioProject |
PRJNA90135 |
| Supplementary data files not provided |
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