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| Status |
Public on Feb 13, 2009 |
| Title |
Epileptogenesis alters gene expression pattern in rats subjected to amygdala-dependent emotional learning |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
Here we tested a hypothesis that epileptogenesis influences expression pattern of genes in the basolateral amygdala that are critical for fear conditioning. Whole genome molecular profiling of basolateral rat amygdala was performed to compare the transcriptome changes underlying fear learning in epileptogenic and control animals. Our analysis revealed that after acquisition of fear conditioning 26 genes were regulated differently in the basolateral amygdala of both groups. Thus, our study provides the first evidence that not only the damage to the neuronal pathways but also altered composition or activity level of molecular machinery responsible for formation of emotional memories within surviving pathways can contribute to impairment in emotional learning in epileptogenic animals. Understanding the function of those genes in emotional learning provides an attractive avenue for identification of novel drug targets for treatment of emotional disorders after epileptogenesis-inducing insult.
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| Overall design |
Experiment description:
Array: Rat Genome 230-2.0 (Affymetrix).
Samples: 2 biological replicates from 4 experimental groups (control unpaired = CU, CU1, control paired = CP, CP1, epileptogenic unpaired = EU, EU1, epileptogenic paired = EP, EP1).
Animals&model: Amygdala stimulation model (Nissinen et al., 2000):
control (C) animals = operated, not stimulated;
epileptogenic (E) animals = stimulated, responded with "good" status epilepticus (at least 40HAFDs within first 3h of SE), not expressing spontaneous seizures through the whole study.
All animals received 5 habituation session to the fear conditioning apparatus lasting 10 min, starting on day 5 (2 sessions on day 5 and 6 and 1 session on day 7) after stimulation (induction of SE).
On 8th day after stimulation:
unpaired (U) = twice received: 2min in apparatus → footshock (1.5mA, 1s)→ tone (75dB for 20 sec)
paired (P) = twice received: 2min in apparatus → tone (75dB for 20 sec) co-terminated by footshock (1.5mA, 1s).
RNA isolation: decapitation → ipsilateral temporal lobe isolated, embed in OCT and frozen in -70C. Cut into 10µm sections, thionin stained (Ambion protocol), basal and lateral nuclei of the amygdala laser-microdissected; total cellular RNA isolated with PicoPure RNA isolation kit (Arcturus).
RNA amplification: 30ng of of total cellular RNA from each rat underwent of 2 rounds of amplification using MessageAmp II aRNA kit (Ambion), according to manufacturer protocol (in vitro transcription time 14h in both rounds).
Sample pooling: aRNA from 2 animals was pooled (10µg in total = 2x5µg) and 8 samples were labeled and hybridized: CP, CP1, CU, CU1, EP, EP1, EU, EU1; (altogether aRNA from 16 rats was used).
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| Contributor(s) |
Majak K, Dabrowski M, Pitkanen A |
| Citation(s) |
19166914 |
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| Submission date |
Nov 17, 2008 |
| Last update date |
Jul 31, 2017 |
| Contact name |
Katarzyna Majak |
| E-mail(s) |
[email protected]
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| Phone |
+48 58 3491401
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| Fax |
+48 58 3491421
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| Organization name |
Medical University of Gdansk
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| Department |
Department of Anatomy and Neurobiology
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| Street address |
Debinki 1
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| City |
Gdansk |
| ZIP/Postal code |
80-211 |
| Country |
Poland |
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| Platforms (1) |
| GPL1355 |
[Rat230_2] Affymetrix Rat Genome 230 2.0 Array |
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| Samples (8)
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| GSM343137 |
control_unpaired_group__replicate_A |
| GSM343275 |
control_unpaired_group__replicate_B |
| GSM343276 |
control_paired_group__replicate_A |
| GSM343277 |
control_paired_group__replicate_B |
| GSM343278 |
epileptogenic_unpaired_group__replicate_A |
| GSM343279 |
epileptogenic_unpaired_group__replicate_B |
| GSM343280 |
epileptogenic_paired_group__replicate_A |
| GSM343281 |
epileptogenic_paired_group__replicate_B |
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| Relations |
| BioProject |
PRJNA110653 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE13624_RAW.tar |
50.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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