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| Status |
Public on Feb 11, 2020 |
| Title |
Genome-wide analysis reveals a switch in the translational program upon oocyte meiotic resumption |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Examination of ribosome-bound mRNA at five timepoints (in duplicates) during oocyte meiosis (prophase I (0 hrs), early pro-metaphase I (2 hrs), pro-metaphase I (4 hrs), late pro-metaphase I (6 hrs), and late metaphase I (8 hrs)).
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| Overall design |
Mouse oocytes were isolated and cultured in vitro. 200 oocytes were collected at various time points during meiotic resumption (prophase I (0 hrs), early pro-metaphase I (2 hrs), mid pro-metaphase I (4 hrs), late pro-metaphase I (6 hrs), metaphase I (8 hrs)). RiboTag-IP was performed and ribosome-bound mRNA was extracted along with total RNA. cDNA libraries were constructed and RNA-Seq was performed. Experiment was performed in duplicates.
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| Contributor(s) |
Luong XG, Daldello E, Conti M |
| Citation(s) |
31970406 |
| |
| Submission date |
Aug 07, 2019 |
| Last update date |
Feb 11, 2020 |
| Contact name |
Xuan Luong |
| Organization name |
UCSF
|
| Street address |
505 Parnassus Ave
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (20)
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| Relations |
| BioProject |
PRJNA559169 |
| SRA |
SRP217720 |
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