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Series GSE135122 Query DataSets for GSE135122
Status Public on Oct 06, 2020
Title Modulation of Syndecan-1/4 reduces the invasive potential of endometrioma through TGF-β signalling
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary We report that modulation of high SDC -1/-4 within certain endometriomas in women having ovarian endometriosis, reversed its higher invasion potential and downregulated several molecules incolved in cancer development.
 
Overall design Endometrioma stromal cells were transiently silenced for SDC-1 andSDC-4, followed by TGF-β stimulation in vitro within 3D-invasion assay. RNA sequnecing were perfomed on harvested spheroids from 3D invasion assay (with/without TGF-β stimulation or SDC-1 or SDC4 silencing) using Smart-seq2 protocol , tagmentation via Nextera kit and sequenced using Nextseq 550. Data were analysed using Partek Flow Genomic analysis software.
 
Contributor(s) Ponandai-Srinivasan S, Bogavarappu NR
Citation(s) 32897364
Submission date Jul 30, 2019
Last update date Oct 06, 2020
Contact name Nageswara Rao Boggavarapu
E-mail(s) [email protected]
Organization name Karolinska Institutet
Department Women and Children health
Lab Bioclinicum J9:30
Street address Visionsgatan 4
City Solna
State/province stockholm
ZIP/Postal code 17164
Country Sweden
 
Platforms (1)
GPL21697 NextSeq 550 (Homo sapiens)
Samples (42)
GSM3986294 Sample 1 with no silencing and no TGF-β stimulation [SA1_S46]
GSM3986295 Sample 2 with no silencing and no TGF-β stimulation [SA2_S47]
GSM3986296 Sample 3 with no silencing and no TGF-β stimulation [SA3_S48]
Relations
BioProject PRJNA557473
SRA SRP216853

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE135122_TGFB_Endo_Normalizedmatrix.txt.gz 1.3 Mb (ftp)(http) TXT
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Processed data are available on Series record
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