NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE133256 Query DataSets for GSE133256
Status Public on Oct 28, 2020
Title Ascending colon-derived mucin-type O-glycans form key mucus layers encapsulating microbiota in the colon (16S rNA-seq)
Organism mouse gut metagenome
Experiment type Other
Summary The colon contains a dense metabolically potent microbiota. The colonic O-glycan-rich mucus has been recognized as a key barrier to prevent microbial intrusion, but how this system forms and functions remains unclear. Here, we discovered that the colon mucus is mainly forged by microbiota-dependent secretion of O-glycosylated Muc2 by goblet cells in the ascending colon, where it seamlessly encapsulates the fecal materials including the microbiota. Deletion of O-glycans in the ascending colon impaired the segregating function of the mucous coating, leading to altered structure and metabolic output of the microbiota, and transcriptional homeostasis of the entire host mucosa. These findings represent a paradigm change of the prevailing model of the colon mucus system and provides new insights into host and microbiota symbiosis.
 
Overall design 16S amplicon sequencing was performed on mouse colon samples derived from the ascending and descending colon. Differential microbial abundance was compared between 3 Groups of Healthy Control and O-glycan mutant littermates that were all colonized with the same microbiota
 
Contributor(s) Bergstrom K, Shan X, Casero D, Batushansky A, Lagishetty V, Jacobs JP, Hoover C, McDaniel JM, McGee S, Houchen CW, Rao C, Griffin T, Sonnenburg JL, McEver RP, Braun J, Xia L
Citation(s) 33093110
NIH grant(s)
Grant ID Grant title Affiliation Name
R01 DK085691 Role of Mucin-type O-glycans in Intestinal Inflammation OKLAHOMA MEDICAL RESEARCH FOUNDATION Lijun Xia
Submission date Jun 25, 2019
Last update date Jan 27, 2021
Contact name David Casero
E-mail(s) [email protected]
Phone 3108250274
Organization name UCLA
Department Pathology and Laboratory Medicine
Street address 610 Charles Young Drive East
City Los Angeles
State/province CA
ZIP/Postal code 90095
Country USA
 
Platforms (1)
GPL22735 Illumina HiSeq 2500 (mouse gut metagenome)
Samples (30)
GSM3904232 Group1-Desc-Control-1
GSM3904233 Group1-Desc-Control-2
GSM3904234 Group1-Desc-TMDKOAsc-1
This SubSeries is part of SuperSeries:
GSE133257 Ascending colon-derived mucin-type O-glycans form key mucus layers encapsulating microbiota in the colon
Relations
BioProject PRJNA550526
SRA SRP211901

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE133256_ProDis_ASVcounts.txt.gz 23.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap