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| Status |
Public on Mar 12, 2021 |
| Title |
UBB+1-induced activation of autophagy reduces cytotoxicity of amyloid-β in yeast |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
UBB+1 is a mutated version of ubiquitin B caused by a transcriptional frameshift. The accumulation of UBB+1, has been linked to ubiquitin-proteasome system (UPS) dysfunction and neurodegeneration. Alzheimer’s disease (AD) is the most common form of neurodegeneration and accumulation of amyloid β (Aβ) in the brain is a prominent neuropathological feature of AD. In our previous study, we found that low expression of UBB+1 could protect cells against several stresses during chronological aging. Here, we applied the genome-wide expression analyses and found that low UBB+1 expression activated the autophagy pathway. Low UBB+1 expression was shown to upregulate vacuolar activity and promote transport of the ATG8p (autophagic marker) to the vacuole. To further study the effects, we expressed low level of UBB+1 in our humanized yeast Aβ models with the expression of either Aβ42 or Aβ40. Interestingly, the co-expression of UBB+1 with Aβ42 or Aβ40 showed a reduction of intracellular Aβ levels and increased chronological life span. In the autophagy deficient mutant background (atg1∆), the intracellular Aβ levels were not affected by UBB+1 expression. Our findings suggest a mechanism of UBB+1 action in reducing intracellular levels of Aβ.
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| Overall design |
The goal of the present study is to evaluate the impact of low expression level of UBB+1 on yeast physiology and transcriptome profiles following the growth phases. Saccharomyces cerevisiae expressing empty plasmid (control strain) and p413 TEF UBB+1 plasmid (LUBB+1 strain) respectively was cultivated in shake flask at 30℃. Samples were taken from duplicate cultures during exponential phase (EX) and day 6 (D6).
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| Contributor(s) |
Chen X, Muñoz-Arellano AJ, Petranovic D |
| Citation(s) |
34751669 |
| |
| Submission date |
Apr 11, 2019 |
| Last update date |
Dec 07, 2021 |
| Contact name |
Xin Chen |
| E-mail(s) |
[email protected]
|
| Organization name |
Chalmers University of Technology
|
| Department |
Department of Biology and Biological Engineering
|
| Street address |
Kemigården 4
|
| City |
Göteborg |
| ZIP/Postal code |
41296 |
| Country |
Sweden |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (8)
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| Relations |
| BioProject |
PRJNA532446 |
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