|
| Status |
Public on Jun 25, 2019 |
| Title |
Glucose metabolism drives epigenetic landscape transitions that dictate muscle stem cell function |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
We report the application of chromatin immunoprecpitation (ChIP) and assay for transposase accessible chromatin (ATAC) followed by sequencing to assay chromatin response to metabolic perturbation.
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| |
|
| Overall design |
Primary myoblasts were treated with dichloroacetate (DCA) for 24 hours, which increases the contribution of glucose to histone acetylation, as well as overall acetylation. ChIP and ATAC-seq was performed to establish the genomic specificity of this response, as well as the resulting effect on chromatin accesibility.
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| |
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| Contributor(s) |
Blau H, Yucel N |
| Citation(s) |
31242425 |
| |
| Submission date |
Apr 03, 2019 |
| Last update date |
Jul 05, 2019 |
| Contact name |
Arndt Siekmann |
| Organization name |
UNIVERSITY OF PENNSYLVANIA
|
| Department |
Perelman School of Medicine
|
| Lab |
Arndt Siekmann
|
| Street address |
421 Curie Boulevard, 1114 Biomedical Research Building II/III
|
| City |
PHILADELPHIA |
| State/province |
Pennsylvania |
| ZIP/Postal code |
19103 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
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| Relations |
| BioProject |
PRJNA530679 |
| SRA |
SRP190375 |
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