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| Status |
Public on Mar 25, 2020 |
| Title |
Transcriptomic changes in Arabidopsis thaliana upon the expression of mutated TFIIS |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The expresion of dominant negative version of Arabidopsis TFIIS (TFIISmut) in plants lacking funtional TFIIS leads to severe growth defects. The genome-wide analysis of transcriptomic changes upon TFIISmut expression was performed by RNA-seq to better understand the biological importance of TFIIS. 10DAS seedlings of transgenic lines carring inducible GFP-TFIIS or GFP-TFIISmut transgenes were studied by RNA-seq following plants exposure to 24h b-estradiol or EtOH (mock) induction.
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| Overall design |
Arabidopsis mRNA profiles of 10-day old transgenic plants with inducible expression of GFP-TFIIS or GFP-TFIISmut fusion proteins in tfIIs-1 background were generated by deep sequencing and analysed comparatively with uninduced plants.
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| Contributor(s) |
Antosz W, Grasser K, Begcy K |
| Citation(s) |
32152189 |
| |
| Submission date |
Mar 29, 2019 |
| Last update date |
May 18, 2020 |
| Contact name |
Kevin Begcy |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Florida
|
| Street address |
1535 Fifield Hall
|
| City |
Gainesville |
| State/province |
Florida |
| ZIP/Postal code |
32611 |
| Country |
USA |
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| Platforms (1) |
| GPL17311 |
Illumina HiSeq 1000 (Arabidopsis thaliana) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA529898 |
| SRA |
SRP189895 |
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