|
| Status |
Public on Mar 29, 2019 |
| Title |
Neuron-astrocyte metabolic coupling protects against activity-induced fatty acid toxicity |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Gene expression profiling of cultured glia with low or high levels of lipid droplets
|
| |
|
| Overall design |
We stained lipid droplets (LDs) in cultured rat glia with nile red stain. We seperated glia containing high numbers of LDs using fluorescence activated cell sorting (FACS). We performed mRNA-seq experiments on glia with low or high levels of LDs.
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| |
|
| Contributor(s) |
Ioannou M, Liu Z |
| Citation(s) |
31130380 |
| |
| Submission date |
Mar 28, 2019 |
| Last update date |
Jun 28, 2019 |
| Contact name |
Zhe Liu |
| E-mail(s) |
[email protected]
|
| Organization name |
Howard Hughes Medical Institute
|
| Department |
Janelia Farm Research Campus
|
| Lab |
Liu
|
| Street address |
19700 Helix Dr.
|
| City |
Ashburn |
| State/province |
Virginia |
| ZIP/Postal code |
20147 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (8)
|
| GSM3689320 |
LD_neg_1: Glia with low levels of LDs replicate 1 |
| GSM3689321 |
LD_neg_2: Glia with low levels of LDs replicate 2 |
| GSM3689322 |
LD_neg_3: Glia with low levels of LDs replicate 3 |
| GSM3689323 |
LD_neg_4: Glia with low levels of LDs replicate 4 |
| GSM3689324 |
LD_pos_1: Glia with high levels of LDs replicate 1 |
| GSM3689325 |
LD_pos_2: Glia with high levels of LDs replicate 2 |
| GSM3689326 |
LD_pos_3: Glia with high levels of LDs replicate 3 |
| GSM3689327 |
LD_pos_4: Glia with high levels of LDs replicate 4 |
|
| Relations |
| BioProject |
PRJNA529609 |
| SRA |
SRP189723 |
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