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| Status |
Public on Mar 18, 2020 |
| Title |
Benzo[a]pyrene and dibenzo[def,p]chrysene in 3D primary human bronchial epithelial cells [RNA-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
In this study, we assess potential differences in mechanism of action for two PAHs, benzo[a]pyrene (BAP) and dibenzo-[def,p]chrysene (DBC), in a primary human 3D bronchial epithelial culture (HBEC) model based on short-term biosignatures identified from global transcriptional profiling.
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| Overall design |
Fully differentiated bronchial epithelial cells (n=4 replicates) were treated with 500 µg/ml (19.80 nanomoles) benzo[a]pyrene (BAP), 10 µg/ml (0.35 nanomoles) dibenzo[def,p]chrysene (DBC) or acetone vehicle control for 48 hrs and isolated mRNA were sequenced on Illumina HiSeq 3000.
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| Contributor(s) |
Tilton SC |
| Citation(s) |
31255691, 32890658 |
| |
| Submission date |
Mar 18, 2019 |
| Last update date |
Oct 28, 2020 |
| Contact name |
Susan C. Tilton |
| E-mail(s) |
[email protected]
|
| Organization name |
Oregon State University
|
| Department |
AG Envr & Molec Toxicology
|
| Street address |
1007 ALS Bldg
|
| City |
Corvallis |
| State/province |
OR |
| ZIP/Postal code |
97331 |
| Country |
USA |
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| Platforms (1) |
| GPL21290 |
Illumina HiSeq 3000 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA527812 |
| SRA |
SRP188728 |
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