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| Status |
Public on Apr 27, 2020 |
| Title |
Effects of host miR-138 on herpes simplex virus 1 gene expression in Neuro-2a cells and their dependence of the binding sites on ICP0 mRNA |
| Organisms |
Mus musculus; Human alphaherpesvirus 1 |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We previously showed that miR-138 can repress herpes simplex virus 1 (HSV-1) ICP0 expression by binding to ICP0 mRNA. However, in this study we found that miR-138 can also repress viral gene expression independent of ICP0. Therefore we conducted this RNAseq experiment to assess the effects of miR-138 on expression of each individual viral gene to search for other viral targets of miR-138
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| Overall design |
We transfected Neuro-2a cell with a miR-138 mimic or a scrambled control, and then infected them with wild-type or M138 mutant virus (which contains mutations in the miR-138 binding sites in ICP0 mRNA). For both wild-type and mutant viruses, miR-138 transfected cells were compared with scrambled control transfected cells. Three biological replicates for each condition.
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| Contributor(s) |
Pan D, Coen DM |
| Citation(s) |
33558653 |
| |
| Submission date |
Feb 28, 2019 |
| Last update date |
Mar 16, 2021 |
| Contact name |
Dongli Pan |
| E-mail(s) |
[email protected]
|
| Organization name |
Zhejiang University
|
| Street address |
866 Yuhangtang Road
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| City |
Hangzhou |
| State/province |
Zhejiang |
| ZIP/Postal code |
110058 |
| Country |
China |
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| Platforms (1) |
| GPL26237 |
Illumina NextSeq 500 (Human alphaherpesvirus 1; Mus musculus) |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE127504 |
Identification of herpes simplex virus 1 and host targets of miR-138 by combined RNAseq/PARCLIP |
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| Relations |
| BioProject |
PRJNA524882 |
| SRA |
SRP187092 |
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