|
| Status |
Public on Aug 26, 2019 |
| Title |
Transcriptomic Analysis in Renal T Lymphocytes Exposes Sodium-Independent Dietary Differences in Dahl Salt-Sensitive Rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Background. The Dahl salt-sensitive (SS) rat is an established model of salt-sensitive hypertension and renal damage. Recently, sodium-independent dietary effects were shown to be important in the development of the SS hypertensive phenotype. Compared to Dahl SS/JrHsdMcwi (SS/MCW) rats fed a purified diet (AIN-76A), grain-fed Dahl SS/JrHsdMcwiCrl rats (SS/CRL; Teklad 5L2F) were less susceptible to salt-induced hypertension and renal damage.
Methods. With the known role of the immune system in hypertension, the present study characterized the immune cells infiltrating SS/MCW and SS/CRL kidneys. To further identify distinct molecular pathways between SS/MCW and SS/CRL, transcriptomic analysis was performed via RNA sequencing in T-cells isolated from the blood and kidneys of low and high salt-fed rats.
Results. Following a 3-week high salt (4.0% NaCl) challenge, SS/CRL rats were protected from salt-induced hypertension (116.5±1.2 vs 141.9±14.4 mmHg) and albuminuria (21.7±3.5 vs 162.9±22.2 mg/day) compared to SS/MCW. Additionally, the absolute number of immune cells infiltrating the kidney was significantly reduced in SS/CRL. RNA-seq revealed >50% of all annotated genes in the entire transcriptome to be significantly differentially expressed in T-cells isolated from blood versus kidney. Pathway analysis of significant differentially expressed genes between SS/MCW and SS/CRL renal and circulating T-cells demonstrated salt-induced changes in genes related to inflammation in SS/MCW compared to metabolism-related pathways in SS/CRL.
Conclusions. These functional and transcriptomic T-cell differences between SS/MCW and SS/CRL show that sodium-independent dietary effects may influence the immune response and infiltration of immune cells into the kidney, ultimately impacting susceptibility to salt-induced hypertension and renal damage.
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| Overall design |
5 experimental groups; 4 pools/experimental group; 3 rats/pool
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| Contributor(s) |
Abais-Battad JM, Mattson DL |
| Citation(s) |
31476910 |
| |
| Submission date |
Feb 25, 2019 |
| Last update date |
Apr 27, 2023 |
| Contact name |
Justine M Abais-Battad |
| Organization name |
Medical College of Wisconsin
|
| Street address |
8701 Watertown Plank Road
|
| City |
Milwaukee |
| State/province |
Wisconsin |
| ZIP/Postal code |
53226 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18694 |
Illumina HiSeq 2500 (Rattus norvegicus) |
|
| Samples (40)
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| Relations |
| BioProject |
PRJNA524128 |
| SRA |
SRP186774 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE127000_B_CRLLS_CRLHS_10W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE127000_B_MCWHS_CRLHS_10W.csv.gz |
1.0 Mb |
(ftp)(http) |
CSV |
| GSE127000_B_MCWLS_7W_10W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE127000_B_MCWLS_CRLLS_10W.csv.gz |
1.0 Mb |
(ftp)(http) |
CSV |
| GSE127000_B_MCWLS_MCWHS_10W.csv.gz |
1.0 Mb |
(ftp)(http) |
CSV |
| GSE127000_BvsK_CRLHS_10W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE127000_BvsK_CRLLS_10W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE127000_BvsK_MCWHS_10W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE127000_BvsK_MCWLS_10W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE127000_BvsK_MCWLS_7W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE127000_K_CRLLS_CRLHS_10W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE127000_K_MCWHS_CRLHS_10W.csv.gz |
1.2 Mb |
(ftp)(http) |
CSV |
| GSE127000_K_MCWLS_7W_10W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE127000_K_MCWLS_CRLLS_10W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
| GSE127000_K_MCWLS_MCWHS_10W.csv.gz |
1.1 Mb |
(ftp)(http) |
CSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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