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| Status |
Public on Jan 30, 2019 |
| Title |
Defining genes in the Caulobacter crescentus SpdR regulon |
| Organism |
Caulobacter vibrioides |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We measured steady-state transcript levels in 1) wild-type C. crescentus, 2) an spdR response regulator deletion strain (ΔspdR), and an spdR phosphomimic strain (SpdR(D64E))
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| Overall design |
Caulobacter crescentus wild type strain CB15, ΔspdR and spdR(D64E) strains were grown to mid log phase (OD600 ~0.3) in M2 defined medium containing 0.15% xylose. Cells were rapidly pelleted and resuspended in Trizol for RNA purification and subsequent RNA sequencing.
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| |
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| Contributor(s) |
Reyes-Ruiz L, Fiebig A, Crosson S |
| Citation(s) |
31075103 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R01 GM087353 |
Integrated Control of Caulobacter Cell Physiology by Visible Light and Stress |
UNIVERSITY OF CHICAGO |
Sean Crosson |
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| |
| Submission date |
Jan 28, 2019 |
| Last update date |
Jun 21, 2019 |
| Contact name |
Sean Crosson |
| E-mail(s) |
[email protected]
|
| Phone |
5178845345
|
| Organization name |
Michigan State University
|
| Department |
Dept. Microbiology and Molecular Genetics
|
| Street address |
567 Wilson Rd
|
| City |
East Lansing |
| State/province |
Michigan |
| ZIP/Postal code |
48824 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL22063 |
Illumina HiSeq 4000 (Caulobacter vibrioides) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA517497 |
| SRA |
SRP182712 |
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