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| Status |
Public on Sep 30, 2019 |
| Title |
3’Pool-seq: an optimized cost-efficient and scalable method of whole-transcriptome gene expression profiling (Run0004) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The advent of Next Generation Sequencing has allowed transcriptomes to be profiled with unprecedented accuracy, but the steep costs associated with full-length library sequencing have posed a limit on the accessibility and scalability of the technology. To address these limitations, we developed 3’Pool-seq, a simple, cost-effective, and scalable RNA-seq method that focuses sequencing to the 3’ end of mRNA transcripts.
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| Overall design |
Transcriptomes (4 replicates each) from homozygous GFAP-IL6 mice were compared to those from wild-type C57BL/6 for differential gene expression using different amounts of cDNA input during the tagmentation step of 3'Pool-seq.
In this specific experiment, the standard Illumina Truseq protocol was used for library preparation. The results were used as a reference for comparison to the 3'Pool-seq protocol.
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| Contributor(s) |
Xi HS, Sholder G |
| Citation(s) |
31959126 |
| |
| Submission date |
Jan 24, 2019 |
| Last update date |
Feb 03, 2020 |
| Contact name |
Hualin Xi |
| E-mail(s) |
[email protected]
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| Organization name |
AbbVie
|
| Street address |
34 webb st
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| City |
Lexington |
| State/province |
Massachusetts |
| ZIP/Postal code |
02420 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE125571 |
3’Pool-seq: an optimized cost-efficient and scalable method of whole-transcriptome gene expression profiling |
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| Relations |
| BioProject |
PRJNA516838 |
| SRA |
SRP181852 |
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