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| Status |
Public on Apr 01, 2019 |
| Title |
Analysis of the messenger RNAs in Spores of Bacillus subtilis |
| Organism |
Bacillus subtilis |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Using RNA-seq, we cataloged messenger RNAs in highly purified dormant Bacillus subtilis spores prepared either on plates or in liquid. Almost all of the most abundant spore mRNAs are encoded by genes expressed only in the developing spore late in sporulation under control of the forespore-specific RNA polymerase sigma factor, sG. Given the levels of the ~40 most abundant mRNAs in dormant spores, we calculated the great majority of the low abundance mRNAs can be present in only a small fraction of the spore population.
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| Overall design |
Examination of highly purified spores from B. subtilis strain PS533, a laboratory 168 derivative that contains plasmid pUB110. Dormant spores were prepared either on plates or in liquid.
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| Contributor(s) |
Korza G, Caimano MJ, Setlow P |
| Citation(s) |
30782632 |
| NIH grant(s) |
| Grant ID |
Grant title |
Affiliation |
Name |
| R21 AI139940 |
Borrelia burgdorferi oligopeptide (Opp) transporter control of cellular homeostasis and growth |
UNIVERSITY OF CONNECTICUT HEALTH CENTER |
Justin D Radolf |
| R56 AI029735 |
RpoS Regulation of Borrelia burgdorferi Genes in vivo |
UNIVERSITY OF CONNECTICUT HEALTH CENTER |
Justin D Radolf |
| R21 AI126146 |
Essential role of Hk1/Rrp1 TCS for survival of Borrelia burgdorferi in ticks |
UNIVERSITY OF CONNECTICUT HEALTH CENTER |
MELISSA J CAIMANO |
| R21 AI128379 |
Identification of genes required by Leptospira interrogans for mammalian host adaptation and/or persistence in the rat model |
UNIVERSITY OF CONNECTICUT HEALTH CENTER |
MELISSA J CAIMANO |
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| |
| Submission date |
Jan 23, 2019 |
| Last update date |
Jul 01, 2019 |
| Contact name |
Peter Setlow |
| E-mail(s) |
[email protected]
|
| Organization name |
UConn Health
|
| Department |
MBB
|
| Street address |
263 Farmington Ave.
|
| City |
Farmington |
| State/province |
CT |
| ZIP/Postal code |
06030 |
| Country |
USA |
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| Platforms (1) |
| GPL21373 |
Illumina MiSeq (Bacillus subtilis) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA516686 |
| SRA |
SRP181675 |
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