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| Status |
Public on Oct 01, 2008 |
| Title |
Changes in Saccharomyces cerevisiae mRNA abundance following oxidative stress and DNA damage stress |
| Platform organisms |
Schizosaccharomyces pombe; Saccharomyces cerevisiae |
| Sample organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by array
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| Summary |
We subjected yeast to two stresses, oxidative stress, which under current settings induces a fast and transient response in mRNA abundance, and DNA damage, which triggers a slow enduring response. Using microarrays we performed a conventional quantification of change in mRNA abundance.
Keywords: Two separate time courses
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| Overall design |
We used Affymetrix microarrays to quantify changes in mRNA abundance following oxidative stress (using hydrogen peroxide) and DNA damage stress (using methyl methanesulfonate) during a three-hour time course (0, 30 min, 60 min, 100 min, 140 min, 180 min).
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| Contributor(s) |
Shalem O, Dahan O, Levo M, Martinez MR, Furman I, Segal E, Pilpel Y |
| Citation(s) |
18854817 |
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| Submission date |
Jul 23, 2008 |
| Last update date |
Feb 21, 2017 |
| Contact name |
Ophir Shalem |
| E-mail(s) |
[email protected]
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| Organization name |
Weizmann Inst.
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| Street address |
Hertzel
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| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
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| Platforms (1) |
| GPL2529 |
[Yeast_2] Affymetrix Yeast Genome 2.0 Array |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE12222 |
Transient transcriptional responses to stress are generated by opposing effects of mRNA production and degradation |
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| Relations |
| BioProject |
PRJNA113853 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE12220_RAW.tar |
10.9 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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