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| Status |
Public on Nov 06, 2018 |
| Title |
Downstream effects of YAP examined by RNA-Seq analysis of skin in YAP2-5SA-∆C transgenic mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To obtain insights into the global changes of gene expression in the mouse skin in response to epidermal YAP activity, we performed whole transcriptome sequencing of skin tissue of YAP2-5SA-∆C transgenic mice.
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| Overall design |
RNA was extracted from dorsal neck skin biopsies of three P33 female YAP2-5SA-∆C mice (TG) showing the early onset of the externally visible phenotype, and of three female wild type littermate mice (WT). The six RNA-Seq libraries were sequenced on the Illumina NextSeq platform to produce approximately 30 million, 75 nucleotide paired-end reads per sample. Differential expression analysis was performed using the Bioconductor packages, edgeR and limma (Voom).
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| Contributor(s) |
Corley SM, Mendoza-Reinoso V, Wilkins MR, Beverdam A |
| Citation(s) |
30382077 |
| |
| Submission date |
Oct 12, 2018 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Susan Corley |
| E-mail(s) |
[email protected]
|
| Phone |
+61 02 9385 8853
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| Organization name |
UNSW
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| Department |
Biotechnology & Biomolecular Sciences
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| Lab |
Systems Biology Initiative
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| Street address |
D26, Kensignton Campus
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| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2052 |
| Country |
Australia |
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| Platforms (1) |
| GPL19057 |
Illumina NextSeq 500 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA496058 |
| SRA |
SRP165628 |
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