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| Status |
Public on Nov 29, 2018 |
| Title |
A multi-tissue full lifespan epigenetic clock for mice [II] |
| Sample organism |
Mus musculus |
| Experiment type |
Methylation profiling by high throughput sequencing
Third-party reanalysis
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| Summary |
Human DNA-methylation data have been used to develop highly accurate biomarkers of aging ("epigenetic clocks"). Recent studies demonstrate that similar epigenetic clocks for mice (Mus Musculus) can be slowed by gold standard anti-aging interventions such as calorie restriction and growth hormone receptor knock-outs. Using DNA methylation data from previous publications with data collected in house for a total 1189 samples spanning 193,651 CpG sites, we developed 4 novel epigenetic clocks by choosing different regression models (elastic net- versus ridge regression) and by considering different sets of CpGs (all CpGs vs highly conserved CpGs). We demonstrate that accurate age estimators can be built on the basis of highly conserved CpGs. However, the most accurate clock results from applying elastic net regression to all CpGs. While the anti-aging effect of calorie restriction could be detected with all types of epigenetic clocks, only ridge regression based clocks replicated the finding of slow epigenetic aging effects in dwarf mice. Overall, this study demonstrates that there are trade-offs when it comes to epigenetic clocks in mice. Highly accurate clocks might not be optimal for detecting the beneficial effects of anti-aging interventions.
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| Overall design |
To complement our in-house data DNA-methylation data collection, we retrieved reduced-representation-bisulfite-sequencing (RRBS) and whole genome bisulfite-sequencing (WGBS) data from previous studies that were available on GEO (GSE52268, GSE58129, GSE67507, GSE80672, GSE85251, GSE89275, GSE93957). We re-processed these data using the BS_Seeker2 methylation calling pipeline for the sake of consistency in all the samples used in our study.
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| Contributor(s) |
Horvath S, Thompson MJ |
| Citation(s) |
30348905 |
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| Submission date |
Sep 18, 2018 |
| Last update date |
Nov 29, 2018 |
| Contact name |
Steve Horvath |
| E-mail(s) |
[email protected]
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| Organization name |
University of California, Los Angeles
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| Department |
Human Genetics
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| Lab |
Horvath
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| Street address |
695 Charles E. Young Drive South, Box 708822
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| City |
Los Angeles |
| State/province |
CA |
| ZIP/Postal code |
90095-7088 |
| Country |
USA |
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| This SubSeries is part of SuperSeries: |
| GSE120137 |
A multi-tissue full lifespan epigenetic clock for mice |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSE120136_GSE52268_re-analyzed_Samples_processed_data.tar.gz |
350.0 Mb |
(ftp)(http) |
TAR |
| GSE120136_GSE58129_re-analyzed_Samples_processed_data.tar.gz |
170.4 Mb |
(ftp)(http) |
TAR |
| GSE120136_GSE67507_re-analyzed_Samples_processed_data.tar.gz |
1.6 Gb |
(ftp)(http) |
TAR |
| GSE120136_GSE80672_re-analyzed_Samples_processed_data.tar.gz |
2.4 Gb |
(ftp)(http) |
TAR |
| GSE120136_GSE85251_re-analyzed_Samples_processed_data.tar.gz |
1.2 Gb |
(ftp)(http) |
TAR |
| GSE120136_GSE89275_re-analyzed_Samples_processed_data.tar.gz |
3.0 Gb |
(ftp)(http) |
TAR |
| GSE120136_GSE93957_re-analyzed_Samples_processed_data.tar.gz |
354.2 Mb |
(ftp)(http) |
TAR |
| GSE120136_re-analyzed_Samples.txt.gz |
2.0 Kb |
(ftp)(http) |
TXT |
| Processed data are available on Series record |
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