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| Status |
Public on Jan 31, 2019 |
| Title |
Rbpj expression in regulatory T cells is critical for restraining TH2 responses [spleen Treg and Tconv RbpjWT expression] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The transcriptional regulator Rbpj is involved in T-helper (TH) subset polarization, but its function in Treg cells remains unclear. Here we show that Treg-specific Rbpj deletion leads to splenomegaly and lymphadenopathy despite increased numbers of Treg cells with a polyclonal TCR repertoire. A specific defect of Rbpj-deficient Treg cells in controlling TH2 polarization and B cell responses was observed, leading to the spontaneous formation of germinal centers and a TH2-associated immunoglobulin class switch as well as T-cell polarization into TH2 effector cells. The observed phenotype was environment-dependent and could be induced by infection with parasitic nematodes. Rbpj-deficient Treg cells adopted open chromatin landscapes and gene expression profiles reminiscent of tissue-derived TH2-polarized Treg cells, with a prevailing footprint of transcription factor Gata-3. The over-expression of Ilt3 rendered Rbpj-deficient Treg cells incompatible to specifically restrain TH2 responses, finally leading to severe and fatal lymphoproliferative disease.
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| Overall design |
We isolated spleen cells from Treg Rbpj-deficient animals and wildtype counterparts. Cells were stained with antibodies and cell populations sorted by FACS. Total RNA was isolated and measured with gene expression microarry.
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| Contributor(s) |
Delacher M, Feuerer M |
| Citation(s) |
30962454 |
| |
| Submission date |
Aug 28, 2018 |
| Last update date |
Apr 17, 2019 |
| Contact name |
Michael Delacher |
| E-mail(s) |
[email protected]
|
| Phone |
+49 6131 17 6574
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| Organization name |
University Medical Center of the Johannes Gutenberg-University Mainz
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| Department |
Institute for Immunology
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| Lab |
Immunology
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| Street address |
Langenbeckstrasse 1
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| City |
Mainz |
| State/province |
Rhineland-Palatinate |
| ZIP/Postal code |
55131 |
| Country |
Germany |
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| Platforms (1) |
| GPL17543 |
Illumina MouseWG-6 v2.0 R2 expression beadchip |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE119169 |
Rbpj expression in regulatory T cells is critical for restraining TH2 responses |
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| Relations |
| BioProject |
PRJNA488286 |
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