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| Status |
Public on Oct 24, 2018 |
| Title |
Sox9-Meis1 inactivation is required for adipogenesis, advancing Pref-1+ to PDGFRa+ cells [Sox9 ChIP-Seq] |
| Organism |
Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
Adipocytes arise from commitment and differentiation of adipose precursors in white adipose tissue (WAT). In studying adipogenesis, precursor markers, including Pref-1 and PDGFRα, are used to isolate precursors from stromal vascular fraction of WAT, but the relationship among the markers is not known. Here, we used Pref-1 promoter-rtTA system in mice for labeling Pref-1+ cells and for inducible inactivation of Pref-1 target, Sox9. We show requirement of Sox9 for maintenance of Pref-1+ proliferative, early precursors. Upon Sox9 inactivation, these Pref-1+ cells become PDGFRa+ cells that express early adipogenic markers. Thus, we show for the first time that Pref-1+ cells precede PDGFRa+ cells in the adipogenic pathway and that Sox9 inactivation is required for WAT growth and expansion. Furthermore, we show that, in maintaining early adipose precursors, Sox9 activates Meis1 which prevents adipogenic differentiation. Our study also demonstrates the Pref-1 promoter-rtTA system for inducible gene inactivation in early adipose precursor population.
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| Overall design |
ChIP-Sequencing for genes bound by Sox9 in 3T3-L1 cells
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| Contributor(s) |
Gulyaeva O, Sul H |
| Citation(s) |
30355480 |
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| Submission date |
Aug 15, 2018 |
| Last update date |
Mar 19, 2019 |
| Contact name |
Hei Sook Sul |
| E-mail(s) |
[email protected]
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| Organization name |
UC Berkeley
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| Department |
NST
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| Lab |
220
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| Street address |
Morgan Hall
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| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (2) |
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| This SubSeries is part of SuperSeries: |
| GSE118575 |
Sox9-Meis1 inactivation is required for adipogenesis, advancing Pref-1+ to PDGFRa+ cells |
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| Relations |
| BioProject |
PRJNA486137 |
| SRA |
SRP157984 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE118571_RAW.tar |
667.3 Mb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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