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| Status |
Public on Aug 21, 2018 |
| Title |
Single cell changes in DBZ or DMSO treated cells derived from Apc-mutant organoid cultures |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We made intestinal organoid cultures from Villin-CreERT2;Apcflox/flox mice. Organoids were subjected to 4-OH-Tam treatment, whereafter Apc mutant organoids were selected in R-Spondin free medium. DBZ or DMSO treatment was started on day 3. On day 8, organoids were dissociated to obtain single cells and analyzed using the Chromium Single-cell 3'RNA-sequencing system
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| Overall design |
We analyzed in total 2 samples (1 DBZ and 1 DMSO), both samples contained 3000 cells
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| Contributor(s) |
Högström J, Alitalo K |
| Citation(s) |
30154153 |
| |
| Submission date |
Aug 02, 2018 |
| Last update date |
Mar 08, 2019 |
| Contact name |
Jenny Hogstrom |
| E-mail(s) |
[email protected]
|
| Organization name |
University of Helsinki
|
| Street address |
Haartmaninkatu 8
|
| City |
Helsinki |
| ZIP/Postal code |
00290 |
| Country |
Finland |
| |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA484197 |
| SRA |
SRP156203 |
