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| Status |
Public on Jun 07, 2008 |
| Title |
Ca2+ oscillatory pattern in fertilized mouse eggs affects gene expression and development to term. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The Ca2+ oscillations initiated by the fertilizing sperm (but terminating concomitant with pronucleus formation) apparently ensure that the events constituting egg activation occur in the correct temporal order; early events (e.g., cortical granule exocytosis) require fewer oscillations than later events (e.g., recruitment of maternal mRNA). Whether the Ca2+ signaling events impact long-term development, in particular development to term, is unknown. Using fertilized eggs that have undergone the first few Ca2+ oscillations, we developed procedures that result either in inhibiting or stimulating the natural pattern of Ca2+ signaling of inseminated eggs. Although the incidence of development to the blastocyst stage is unaltered by these procedures, fewer offspring are born following embryo transfer, indicating that developmental competence of the blastocysts is reduced. Interestingly, embryo transfer experiments reveal that when the natural regime of Ca2+ oscillations is precociously interrupted, the incidence of implantation is compromised whereas hyper-stimulation of Ca2+ signaling events compromises post-implantation development. Moreover, although there was no major difference in the overall growth rates of the offspring, those obtained following hyper-stimulation exhibited a far greater variability in their weight. Analysis of global patterns of gene expression by microarray analysis revealed that approximately 20% of the transcripts are mis-regulated when too few oscillations are experienced by the embryo and EASE analysis indicates that genes preferentially involved in RNA processing and polymerase II transcription are differentially affected. In addition, a set of genes involved in cell adhesion is also mis-expressed and could thus be mechanistically linked to the observed reduced implantation. Only about 3% of the transcripts were mis-regulated following hyper-stimulation, and EASE analysis indicates that genes preferentially involved in metabolism are differentially affected. In toto, these results indicate that a range Ca2+ signaling events following fertilization (an excess or reduction) has long-term effects on both gene expression and development to term.
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| Overall design |
We profiled the global gene expression in the blastocysts by treatment of Ca2+ oscillations, and identified the genes differentially expressed.
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| Contributor(s) |
Pan H, Ozil J, Banrezes B, Tóth S, Schultz RM |
| Citation(s) |
16996050 |
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| Submission date |
Jun 04, 2008 |
| Last update date |
Feb 11, 2019 |
| Contact name |
Hua Pan |
| E-mail(s) |
[email protected]
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| Organization name |
University of Pennsylvania
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| Department |
Department of Biology
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| Street address |
415 S University Avenue
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| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19104 |
| Country |
USA |
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| Platforms (1) |
| GPL1261 |
[Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array |
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| Samples (12)
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| GSM296817 |
control blastocyst, replicate 4 |
| GSM296818 |
blastocyst treated by 20 pulses, replicate 1 |
| GSM296819 |
blastocyst treated by 20 pulses, replicate 3 |
| GSM296820 |
blastocyst treated by 20 pulses, replicate 2 |
| GSM296821 |
blastocyst treated by 20 pulses, replicate 4 |
| GSM296822 |
blastocyst cultured by Ca free medium, replicate 1 |
| GSM296823 |
blastocyst cultured by Ca free medium, replicate 2 |
| GSM296824 |
blastocyst cultured by Ca free medium, replicate 3 |
| GSM296825 |
blastocyst cultured by Ca free medium, replicate 4 |
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| Relations |
| BioProject |
PRJNA106093 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE11687_RAW.tar |
56.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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