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Series GSE11685 Query DataSets for GSE11685
Status Public on Nov 10, 2009
Title Translational response following activation of GCN2 versus PERK
Organism Mus musculus
Experiment type Expression profiling by array
Summary In eukaryotes, regulation of mRNA translation enables a fast, localized and finely tuned expression of gene products. Within the translation process, the first stage of translation initiation is most rigorously modulated by the actions of eukaryotic initiation factors (eIFs) and their associated proteins. These 11 eIFs catalyze the joining of the tRNA, mRNA and rRNA into a functional translation complex. Their activity is influenced by a wide variety of extra- and intracellular signals, ranging from global, such as hormone signaling and unfolded proteins, to specific, such as single amino acid imbalance and iron deficiency. Their action is correspondingly comprehensive, in increasing or decreasing recruitment and translation of most cellular mRNAs, and specialized, in targeting translation of mRNAs with regulatory features such as a 5’ terminal oligopyrimidine tract (TOP), upstream open reading frames (uORFs), or an internal ribosomal entry site (IRES). In mammals, two major pathways are linked to targeted mRNA translation. The target of rapamycin (TOR) kinase induces translation of TOP and perhaps other subsets of mRNAs, whereas a family of eIF2 kinases does so with mRNAs containing uORFs or an IRES. TOR targets translation of mRNAs that code for proteins involved in translation, an action compatible with its widely accepted role in regulating cellular growth. The four members of the eIF2 kinase family increase translation of mRNAs coding for stress response proteins such as transcription factors and chaperones. Though all four kinases act on one main substrate, eIF2, published literature demonstrates both common and unique effects by each kinase in response to its specific activating stress. This suggests that the activated eIF2 kinases regulate the translation of both a global and a specific set of mRNAs. Up to now, few studies have attempted to test such a hypothesis; none has been done in mammals.

We use array analysis to determine the global mRNA shift into polysomes following a stress response, and to compare the translational response following activation of GCN2 versus PERK, two of the four eIF2alpha kinases.

This SuperSeries is composed of the SubSeries listed below.
 
Overall design Refer to individual Series
 
Contributor(s) Dang Do AN, Kimball SR, Cavener DR, Jefferson LS
Citation(s) 19509078
Submission date Jun 04, 2008
Last update date Feb 11, 2019
Contact name An Dang Do
Organization name Penn State College of Medicine
Department Cellular and Molecular Physiology
Lab Leonard Jefferson/Scot Kimball
Street address 500 University Drive
City Hershey
State/province PA
ZIP/Postal code 17033
Country USA
 
Platforms (1)
GPL1261 [Mouse430_2] Affymetrix Mouse Genome 430 2.0 Array
Samples (32)
GSM289438 Unfractionated, Gcn2 wild-type liver, +Met, biological rep1
GSM289439 Unfractionated, Gcn2 wild-type liver, +Met, biological rep2
GSM289440 Unfractionated, Gcn2 wild-type liver, -Met, biological rep1
This SuperSeries is composed of the following SubSeries:
GSE11496 Expression data from Gcn2 wild-type and knockout mouse liver perfused with or without methionine
GSE11684 Expression data from Perk wild-type and knockout mouse liver perfused without or with 2,5-di-tert-butylhydroquinone
Relations
BioProject PRJNA106089

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE11685_RAW.tar 116.9 Mb (http)(custom) TAR (of CEL, CHP)
Raw data provided as supplementary file
Processed data included within Sample table
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