NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE116826 Query DataSets for GSE116826
Status Public on Jul 10, 2018
Title sRNA sequencing of liver from lean rats and ZDF rats treated with vehicle or colesevelam
Organism Rattus norvegicus
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Colesevelam is a bile acid sequestrant approved to treat both hyperlipidemia and type 2 diabetes, but the mechanism for its glucose lowering effects is not fully understood. The aim of this study was to investigate the role of hepatic microRNA’s as regulators of metabolic disease and to investigate the link between the cholesterol and glucose lowering effects of colesevelam. To quantify the impact of colesevelam treatment in rodent models of diabetes, metabolic studies were performed in Zucker Diabetic Fatty (ZDF) rats and db/db mice. Colesevelam treatments significantly decreased plasma glucose levels and increased glycolysis in the absence of changes to insulin levels in ZDF rats and db/db mice. High-throughput sequencing and real-time PCR were used to quantify hepatic miRNA and mRNA changes, and the cholesterol-sensitive miR-96/182/183 cluster was found to be significantly increased in livers from ZDF rats treated with colesevelam compared to vehicle controls. In summary, these results support that colesevelam likely improves glycemic control through hepatic miR-96/182/183, a mechanism that directly links cholesterol and glucose metabolism.
 
Overall design Examination of miRNA changes in livers of ZDF rats treated with vehicle or colesevelam
 
Contributor(s) Sedgeman L, Vickers K
Citation(s) 30160993
Submission date Jul 09, 2018
Last update date Oct 22, 2018
Contact name Leslie Roteta Sedgeman
Organization name Vanderbilt University
Street address 2220 Pierce Avenue
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
 
Platforms (1)
GPL18694 Illumina HiSeq 2500 (Rattus norvegicus)
Samples (18)
GSM3262303 ZDF + Colesevelam - Sample 1
GSM3262304 ZDF + Colesevelam - Sample 2
GSM3262305 ZDF + Colesevelam - Sample 3
Relations
BioProject PRJNA480300
SRA SRP152880

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE116826_rat_2570.define_DESeq2.csv.gz 34.3 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap